Mitochondrial Profiling in Cells Under Physiological Conditions and In Response to Stress

Guest Speaker:
Victor Darley-Usmar, Ph.D.
Professor of Pathology,
Director of the Center for Free Radical Biology,
University of Alabama at Birmingham

Wednesday, August 4, 2010
9:30 am San Francisco
12:30 pm Boston
18:30 Copenhagen

Webinar Abstract:

It is well known that mitochondria provide intermediary metabolites and serve the bioenergetic needs of a wide variety of cells. The extracellular flux technology is particularly well suited for assessing how these functions impact on oxidative phosphorylation and glycolysis. A particularly useful and straight-forward protocol has been to add in series an inhibitor of the ATP synthase (oligomycin), an uncoupler (FCCP) and a respiratory chain inhibitor (antimycin or rotenone) to obtain mitochondrial profile of cells under basal and stressed conditions.

In this webinar we demonstrated:

• How this mitochondrial profiling can be used to determine the response of cells to oxidative stress.
• How cellular differentiation can modify the mitochondrial profile.
• How the basal measurement of oxygen consumption can be combined with the measurement of glycolysis to gain a metabolic image of the cells response to stressors.

Assay:

Mitochondrial Function

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