Cardiovascular oxidative stress: Importance of the mitochondrial reserve capacity
Bradford G. Hill, Ph.D.
Assistant Professor of Medicine
Center for Diabetes and Obesity Research
University of Louisville
Louisville, KY
Webinar Abstract:
Reactive oxygen and nitrogen species are part of normal metabolism and play prominent roles in cardiovascular (patho)physiology. Interestingly, these reactive species or their secondary products covalently modify proteins, thereby modulating enzyme function or eliciting redox signaling. Mitochondria, in particular, are prominent sources of such reactive species and are hotspots for oxidative protein modifications. Nevertheless, how reactive species or the protein modifications they induce affect cellular bioenergetics remains unclear. Accordingly, we have used extracellular flux (XF) analysis to understand how reactive species affect cellular bioenergetics.
Join us for this 45-minute webinar to learn how we are employing XF technology to advance our understanding of how oxidative protein modifications can affect bioenergetic pathways in cardiovascular cells.
You Will Learn:
- The different oxidative modifications occurring in the cell, including those that could impact cellular bioenergetics.
- How the responses of cardiovascular cells to reactive species can differ based on cell type.
- How oxidative modifications derived from reactive lipids and ROS/RNS affect bioenergetic function, reserve capacity, and the apparent respiratory state.
Assay:
Mitochondrial Function: BOFA
Log In to View Webinar On-Demand.