How to Measure Mitochondrial Function in Whole Islets.
Sensing the nutrient sensor: measuring bioenergetic parameters from intact mouse and human islets of langerhans.
Orian Shirihai, MD, PhD
Associate Professor
Boston University
Medical Center
Webinar Abstract:
While oxidative phosphorylation in most cells is primarily controlled by the cellular demand for ATP, beta cells are unique in their capacity to increase oxidative phosphorylation in response to fuel availability. As such, in the beta cell, the engine is used as a sensor for fuel.
One significant manifestation of diabetes is the gradual reduction in mitochondrial capacity to produce secretory signals in response to nutrients. Our goal is to understand the mechanisms that underlie deterioration of mitochondrial function during the development of _-cell dysfunction and diabetes. To study mitochondrial function in primary beta cells we have employed and developed techniques that allow for measurement of oxidative phosphorylation and mitochondrial membrane potential in the intact islets.
Join us for this 45 minute webinar to learn how we are employing intact islets from rodent and human to:
- Study oxygen consumption in intact islets from healthy and diabetic mice and humans.
- Study mitochondrial membrane potential changes in response to fuel challenge using different membrane potential probes.
- Discuss and compare the use of the two approaches, membrane potential vs oxygen consumption in addressing hypotheses concerning beta cell bioenergetics.
Assay:
Mitochondrial Function: Glucose, BOFA
Substrate Utilization: Glucose
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