Primary Renal Proximal Tubules as a Platform for Mitochondrial Biogenesis and Toxicity Assays

Guest Speaker:

Craig Beeson, PhD
Associate Professor
Department of Pharmaceutical and Biomedical Sciences
Medical University
of South Carolina

Current in vitro models of mitochondrial biogenesis and toxicity are inadequate. Cultured cells are very glycolytic with minimal aerobic metabolism, and there are limited high-throughput real-time metabolomic assays. Consequently, new cellular models and metabolomic methodologies are needed to evaluate mitochondrial biogenesis and toxicity in a high-throughput modality. We have developed primary cultures of renal proximal tubular cells (RPTC) that exhibit in vivo levels of aerobic metabolism, are not glycolytic, and retain higher levels of differentiated functions.

Join us for the 45-minute webinar to learn how we have validated, using the Seahorse XF analyzer, the primary rabbit RPTC model as a sensitive and precise platform for the evaluation of mitochondrial biogenesis and toxicity.

You will learn:

How we optimized the isolation, and culturing of primary RPTC in a multiwell format as demonstrated by aerobic capacity, coupling of transport to respiration, and substrate utilization.
How we validated the primary RPTC assay by confirming the activity of agents known to stimulate mitochondrial biogenesis such as AICAR, metformin, and resveratrol.
How the assay also identifies known nephrotoxicants, such as gentamycin, cisplatin, and mercury while excluding non-nephrotoxicants such as mitomycin C.