Seahorse Bioscience

Achieving higher throughput and greater content when measuring mitochondrial function

Guest Speaker:
David Ferrick
David Ferrick
VP, Biology & Applications,
Seahorse Bioscience, Inc.

Webinar Abstract:

The introduction of Extracellular Flux (XF) technology in 2006 enabled non-invasive, real time profiling of cellular bioenergetics and mitochondrial function in lving cells. This opened a new window to study cellular metabolism. And the ability to assess primary, engineered and genetically altered cells under various substrates conditions or drug exposures became easier.

Now, over 200 scientists routinely rely on Seahorse XF analyzers. With the advent of the 96-well based system, screening for compounds or pathways that alter mitochondria function and overall bioenergetic behavior can now be routinely accomplished. As well, basic studies can benefit by the additional throughput so that many more conditions such as multiple substrates and genetic alterations can be run in a single experiment.

Join us for this 45-minute webinar to learn about generating better experiments with the XF96 and how it is used to assess mitochondrial dysfunction and cellular bioenergetics with a level of throughput and data quality never seen before.

You will learn:

  • How easy it is to generate 96 wells of cellular bioenergetic data.
  • How precise and accurate XF data is.
  • How to take advantage of the much greater throughput in designing bioenergetic experiments.

Assay:

Energy Expenditure: Flux: Mitochondrial Respiration; Glycolysis
Mitochondrial Function: BOFA

 

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Originally presented Thursday, January 22, 2009


References and Additional Links:

Medical University of South Carolina

Beeson Research Group


Center for Cell Death, Injury & Regeneration

MUSC/Seahorse Bioscience Academic Core Facility


Orian Shirihai Lab

Mitochondrial ARC

Selected Publications

1. Advances in Measuring Cellular Bioenergetics using Extracellular Flux. Ferrick DA, Neilson A, and Beeson C. (2008). Drug Discov Today, 2008 Mar;13(5-6):268-74. Epub 2008 Feb 13.

2. Multiparameter Metabolic Analysis Reveals a Close Link between Attenuated Mitochondrial Bioenergetics and Enhanced Glycolysis Dependency in Human Tumor Cells.". Wu M, Neilson A, Swift AL, Moran R, Tamagnine J, Parslow D, Armistead S, Lemire K, Orrell J, Teich J, Chomicz S and Ferrick DA. (2007). Am J of Physiol Cell Physiol 292:C125-C136.