The Response of the Mitochondrion to Oxidative Stress in Cardiovascular Cells

Guest Speaker:
Victor Darley-Usmar, Ph.D.
Professor of Pathology,
Director of the Center for Free Radical Biology,
University of Alabama at Birmingham

Webinar Abstract:

It is well known that the capacity for mitochondrial respiration exceeds what is needed to satisfy the basic metabolic demands of the cell. It has been generally assumed that this so called "reserve capacity" is drawn upon when cells are subjected to stress including the reactive oxygen and nitrogen species (ROS/RNS).

This is particularly important in the cardiovascular system in which a diverse range of cells are exposed to reactive oxygen and nitrogen species in association with pathologies such as atherosclerosis, hypertension and diabetes.

In this webinar we demonstrated:

• How mitochondrial reserve capacity is used by vascular cells to protect themselves against oxidative stress.
• How mitochondria in a cellular context respond to oxidative stress.
• Why this is potentially important since mitochondrial reserve capacity can be decreased by exposure to environmental toxins, aging or the acute effects of disease.

Assay:

Mitochondrial Function: BOFA

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Originally presented November 11, 2008

Selected Publications

1. Landar, A., Shiva, S., Levonen, A. L., Oh, J. Y., Zaragoza, C., Johnson, M. S., and Darley-Usmar, V. M. Induction of the permeability transition and cytochrome c release by 15-deoxy prostaglandin J2 in mitochondria. Biochem J 394:185-95 (2006).

2. Gutierrez J, Ballinger SW, Darley-Usmar VM, and Landar A. Free radicals, mitochondria, and oxidized lipids: the emerging role in signal transduction in vascular cells. Circ Res. 99: 924-932, 2006.

3. Landar A, Zmijewski JW, Dickinson DA, Le Goffe C, Johnson MS, Milne GL, Zanoni G, Vidari G, Morrow JD, and Darley-Usmar VM. Interaction of electrophilic lipid oxidation products with mitochondria in endothelial cells and formation of reactive oxygen species. American journal of physiology 290: H1777-1787, 2006.

4. Bailey, S.M., Landar, A., Darley-Usmar, V. Mitochondrial proteomics in free radical research. Free Radic Biol Med. 38:175-88, 2005.

5.Venkatraman, A., Landar, A., Davis, A. J., Chamlee, L., Sanderson, T., Kim, H., Page, G., Pompilius, M., Ballinger, S., Darley-Usmar, V., and Bailey, S. M. (2004) Modification of the mitochondrial proteome in response to the stress of ethanol-dependent hepatotoxicity. J Biol Chem 279, 22092-22101