Tutorial 2: Measuring Bioenergetics in Primary Cell Types

Webinar Abstract:

Recent discoveries have linked mitochondrial dysfunction and defects in cellular bioenergetics to the pathophysiology of many diseases including obesity, diabetes, cancer, cardiomyopathy and neurodegeneration. The introduction of Extracellular Flux (XF) technology has enabled non-invasive, real time profiling of cellular bioenergetics and mitochondrial function in living cells, opening a new window to study these diseases.

You Will Learn:

• How to prepare primary cell populations from different tissues for XF analysis
• How to assess bioenergetic profiles of primary cell populations
• How to link bioenergetic profiles to relevant cellular physiology

Assay:

Energy Expenditure: Shift between respiration and glycolysis

 

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Originally presented August 27, 2008

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References and Additional Links

Medical University of South Carolina

Beeson Research Group

Center for Cell Death, Injury & Regeneration

MUSC/Seahorse Bioscience Academic Core Facility

Orian Shirihai Lab

Mitochondrial ARC

Selected Publications

1. Advances in Measuring Cellular Bioenergetics using Extracellular Flux. Ferrick DA, Neilson A, and Beeson C. (2008). Drug Discov Today, in press.

2. Multiparameter Metabolic Analysis Reveals a Close Link between Attenuated Mitochondrial Bioenergetics and Enhanced Glycolysis Dependency in Human Tumor Cells.". Wu M, Neilson A, Swift AL, Moran R, Tamagnine J, Parslow D, Armistead S, Lemire K, Orrell J, Teich J, Chomicz S and Ferrick DA. (2007). Am J of Physiol Cell Physiol 292:C125-C136.