Tutorial: Measuring Bioenergetics in a Variety of Cell Types
Guest Speaker:
David Ferrick
VP, Biology & Applications,
Seahorse Bioscience, Inc.
Webinar Abstract:
Recent discoveries have linked mitochondrial dysfunction and defects in cellular bioenergetics to the pathophysiology of many diseases including obesity, diabetes, cancer, cardiomyopathy and neurodegeneration. The introduction of Extracellular Flux (XF) technology has enabled non-invasive, real time profiling of cellular bioenergetics and mitochondrial function in living cells, opening a new window to study these diseases.
Also discussed:
- How to prepare different cell types for XF analysis
- How to assess bioenergetic profiles of various cell types
- How to link bioenergetic profiles to cellular physiology
Assay:
Mitochondrial Function: BOFA (Glucose,) Glycolytic Compensation, FCCP
Substrate Utilization: Glucose, Palmitate, Etomoxin
Log In to View on-DemandOriginally presented July 17, 2008
References and Additional Links
Medical University of South Carolina
Beeson Research Group
Buck Institute for Age Research
The Nicholls Lab
Contact Information
David Ferrick, Ph.D
VP, Biology & Applications, Seahorse Bioscience, Inc.
Email: dferrick@seahorsebio.com