Understanding When and How to Use Intact Cells and Isolated Mitochondria in Investigating Bioenergetics

Advanced XF Training - Course information

Join us for an exceptional opportunity, one-on-one training in an intimate setting with renowned cellular bioenergeticists, David Nicholls and Martin Brand.

Date:

April 3-5 & April 10-12, 2012

Location:

Buck Institute for Research and Aging
8001 Redwood Blvd.
Novato, California 94945

Instructors:

David Nicholls, PhD, FSRE & Martin Brand, PhD

David Nicholls' research has focused on the function and physiology of mitochondria in intact cells, with an emphasis on their roles in the life and death of stressed neurons, and also on the understanding of the mechanism of insulin secretion and its failure in type-one diabetes. Martin Brand's research focuses on the understanding of how the function of mitochondria within cells is regulated to give the exquisite control of nutrient use and energy turnover that is characteristic of healthy cells.

In this 2 ½ - 3 day advanced training course, you will work with these renowned cellular bioenergetic researchers, delving into the depths of bioenergetics and mitochondrial function, learning how its regulation and effects on cells can be described quantitatively, and of how we might be able to alter it to affect conditions such as diabetes, obesity, degenerative diseases and aging. Experience with the XF Analyzer, or other respirometry methods such as the Clark Electrode, and familiarity with designing and performing bioenergetic experiments is recommended.

You will learn:

How to design and perform XF assays with isolated mitochondria and whole cells to obtain valuable bioenergetic information
How to review and interpret XF data, and understand its far reaching implications to your research
The Ins and Outs of Isolating Mitochondria (Optional ½ day on Day 3)

Course Outline

Day 1	9:00am-5:00pm	Understanding When and How to Use Intact Cells and Isolated Mitochondria in Investigating Bioenergetics: Isolated Mitochondria (wet lab, lectures, XF data discussions)
Day 2	9:00am-5:00pm	Understanding When and How to Use Intact Cells and Isolated Mitochondria in Investigating Bioenergetics: Intact Cells (lectures, XF data discussions)
Day 3	9:00am-12:00pm	XF Case Studies Discussion Cost: $1,250
Day 3	1:00pm-5:00pm	The Ins and Outs of Isolating Mitochondria This additional, optional session will provide an overview on methods for isolation of mitochondria, and will include both a wet lab demonstration, as well as a lecture. Additional Cost: $250 (Limited to 8 students)

Hotel Recommendation

Best Western Novato Oaks Inn
Call the front desk, 415-883-4400, and mention the phrase "Buck Training – April" for a discount rate of $119 per night

Registration:

The Advanced XF Training Course includes class materials, labs, lectures, XF protocols, XF data analysis software, as well as lunches, dinners and ground transportation between the Buck Institute and hotel. A USB drive containing wet lab data and electronic copies of all course presentations will be provided to each student to take with them. The course fee does NOT include hotel accommodations or travel to and from Novato, CA.

Cancellations and Disclaimer:

We make every possible effort to run the training courses as described. However, Seahorse reserves the right to cancel courses at any time if they prove to be non-viable, or for any reason which lies outside Seahorse's control, and to alter dates and times and reschedule classes if this becomes necessary.