SimCell™ Publications
Application Notes
Automated Cell Culture Process Development: New Strategies with High Throughput MicroBioreactor Technology
H. Brett Schreyer, Scott E. Miller and Seth Rodgers, Gen. Eng. News, Vol.27, No.17, p.44
SimCell™ MicroBioreactor technology is shown to be a predictive scale-down model for stirred-tank bioreactor systems. New workflow strategies are described to take advantage of the high-throughput, parallel nature of the multiplexed MicroBioreactor platform.
SimCellT Robotics System
The characteristics and features of the SimCell™ Robotics System.
Culture of Mammalian Cells in SimCellT MicroBioreactors
The use of SimCell™ MicroBioreactor Arrays (MBAs) for the development and optimization of therapeutic recombinant protein production processes using mammalian cell cultures.
Small Volume Off-Line Analyses Compatible with the SimCellT Robotics System
Several analytical methods/platforms that are compatible with the SimCell™ Robotics System.
Best Practices for pH Control in the SimCellT System
The pH measurement and control methods used on the SimCell™ Robotics System.
Glucose Measurement and Control in the SimCellT System
Method for glucose measurement using small volume samples removed from the SimCell™ MicroBioreactor Arrays (MBAs).
Dissolved Oxygen Measurement and Control in the SimCellT System
Dissolved oxygen (DO) measurement and control methods used on the SimCell™ Robotics System.
Posters
Accelerated Development Timelines by Simultaneous Clone and Process Selection using the SimCell™ System
A. Peter Russo, BioProcessors Corp., Woburn, MA
The ability of the SimCell™ System to conduct clone screening experiments using complex fed-batch processes with measurement and control capabilities similar to large-scale bioreactors can be leveraged to perform optimization in parallel. This not only results in significant time savings but can also identify interactions between clone and process that might otherwise be missed.
Applications of the SimCell™ System as a High-Throughput MicroBioreactor Platform
H. Brett Schreyer, Rachel Legmann, A. Peter Russo, BioProcessors Corp., Woburn, MA and Rodney Combs, Pfizer, Inc., St. Louis, MO
Cell growth and productivity of a CHO cell line producing a monoclonal antibody were optimized using the SimCell™ System. The effects of feed, pH set point and temperature shift on IVCC, terminal titer and specific productivity were examined.
Scale-Down Process Optimization using the SimCell™ System: An Automated, High-Throughput Microbioreactor Platform
H. Brett Schreyer, Rachel Legmann, A. Peter Russo, BioProcessors Corp., Woburn, MA and Rodney Combs, Pfizer, Inc., St. Louis, MO
Growth and productivity of a monoclonal antibody producing CHO cell lines were optimized using the SimCell™ System. The best condition from the DOE was scaled to a bench-scale bioreactor for verification of the results. Process measurement and control was also found to be similar between the two systems.
Predicting Bioreactor Cell Culture Performance with a Sub-Milliliter MicroBioreactor System
H. Brett Schreyer, Rachel Legmann, BioProcessors Corp., Woburn, MA
A model CHO cell line producing human interferon-y is used to compare culture performance between bench bioreactors and SimCell™ MicroBioreactors. Specifically, cell growth, glucose metabolism, glutamine metabolism, interferon production, and interferon gylcosylation are examined and compared for both systems. Results demonstrate equivalency between the two systems, providing evidence that the SimCell™ MicroBioreactor scale-down model can predict performance of conventional stirred-tank bioreactors.
New Strategies for Effective Process Development Using a High Throughput Automated MicroBioreactor System
H. Brett Schreyer, Scott Miller, Tim Johnson, Seth Rodgers, BioProcessors Corp., Woburn, MA
Experimental results show excellent correlation between SimCell™ MicroBioreactor and benchtop bioreactor cell cultures. Optimum conditions indicated by the SimCell™ MicroBioreactor model were confirmed in 3L bioreactors, providing evidence of the scale-up predictability of the SimCell™ platform.
Implementation of High Throughput Systems for Media and Process Development
Soverin Karmiol, Mykle Gaynor, Robert Kenerson, John Daley, Rich Hassett, Steven Peppers, Invitrogen Corporation, Grand Island, NY
The SimCell™ System is implemented in a media and process development workflow to investigate 196 individual combinations of media components, feed regimens and process conditions. Interactions and effects of these factors on the growth of a CHO cell clone are studied. Results illustrate how the SimCell™ integrates into a media development and optimization workflow.
Evaluation of a Novel MicroBioreator System for Cell Culture Optimization
G. Wilson, Novo Nordisk A/S, Kobenhavn, Denmark
A statistical factorial experiment was conducted to examine the effect of pH and temperature shift on a bioproduction process to produce a therapeutic protein from CHO cells. Compared to the one year required to study 50 different combinations of pH and temperature using conventional benchtop fermenters, only one month would be required using an automated SimCell™ System. Specific productivity results from the SimCell™ MicroBioreactors correlated well with those from 5-L fermenters.
Novel MicroBioreactors as Scale-down Simulators of Large Stirred-Tank Reactors
Timothy J. Johnson, A Peter Russo, Brian O. Benoit and George J. Vella
Computational fluid dynamic models were developed to ensure that the fluidic micro environment of the SimCell™ MicroBioreactor is similar to stirred tanks. Shear range and shear frequency in the SimCell™ MicroBioreactor are compared to shake flasks and bioreactors. Results indicate that the SimCell™ MicroBioreactor is representative as a scale-down bioreactor model.