XF96 Consumables

The Seahorse XF Extracellular Flux Analyzer utilizes four types of consumables:

• XF96 Assay Kits
• XF96 Cell Culture Microplates
• XF96 Islet Capture Microplates
• XF Calibrant Solution
• XF Assay Medium

XF96 FluxPaks

FluxPaks simplify your ordering process providing the correct ratio of XF Assay Kits to XF Cell Culture Plates and XF Calibrant required to perform XF assays.

XF Assay Medium is optional and must be ordered separately.

XF96 FluxPak with Polystyrene Microplates: For standard cell culture surface applications.

One FluxPak contains supplies to assay eighteen 24-well microplates.

Part Number: 10085-001

XF96 FluxPak with PET Microplates: For low gas permeable cell culture surface and maximal ECAR applications.

One FluxPak contains supplies to assay eighteen 24-well microplates.

Part Number: 10085-001

XF96 Islet Capture FluxPak

One FluxPak contains supplies to assay six 96-well islet capture microplates.

Part Number: xxxxx-100

XF96 Assay Kit

(available only in FluxPaks)

The XF Assay Kit consists of a disposable, 96-well, dual-analyte sensor cartridge, calibration plate, and lid. The sensor cartridge measures OCR (oxygen consumption rate) and ECAR (extracellular acidification rate).

The two fluorophores for analyte detection are embedded in a polymer spotted on the end of each of the sensor sleeves. The OCR is determined by the amount that oxygen quenches the fluorescence of a fluorescein complex. The ECAR sensor responds to changes in proton (H+) concentration as they diffuse across the fluorophore based polymetric substrate. The negative log of proton concentration is equal to the pH. The XF Analyzer's fiber optic wave guide is inserted into the sleeves of the sensor cartridge. Light is pumped through the fiber optic bundles, exciting the fluorophores and the resultant emission is detected and measured.

The sensor cartridge contains barcode information matching it to its corresponding tissue culture plate. The disposable assay kit may be used for hours or days. Sensor cartridges are not able to analyze non matching cell culture plates.

Each sensor cartridge also contains two compound storage and delivery ports per well. A unique multi-channel drug delivery system injects compounds into each cell well on command. This allows multiple measurements to be made using a single population of cells, and increases the effective throughput of the assay plate.

XF assay kits are supplied in individual containers.

XF Assay Kits may only be purchased as part of a FluxPak.

XF96 Cell Culture Microplates

XF96 cell culture microplates are designed to maximize the sensitivity of the sensor cartridge measurements.

Molded stops on the bottom of the plate prevent the sensor cartridge sleeves from contacting or disrupting the cell monolayer. Cells are not affected by the measurement process and can be reused.

XF96 cell culture microplates are tissue culture treated for optimum adherence and growth of mammalian cells. The XF microplates are supplied in individual gamma irradiated containers, and are packaged ten per box.

Two types of XF96 Cell Culture Microplates are available:

XF96 Polystyrene Cell Culture Microplates: with a standard cell culture surface, designed for most cell types and applications.

Part Number: 101085-004

XF96 PET Cell Culture Microplates : molded from Polyethylene Terephthalat.  PET does not absorb CO2, so PET plates equilibrate faster and often provide improved ECAR measurements.

Part Number: 101104-004

XF96 Islet Capture Microplates

(Part #xxxxxx-100)

XF96 islet capture microplates are designed to assay the cellular metabolism of pancreatic islets with an XF96 Analyzer.

The Islet Capture Microplate uses a unique capture screen to keep islets an optimal distance from the XF sensor while permitting the diffusion of compounds and allowing for the free exchange of nutrients and gasses to keep cells healthy throughout the assay.

The XF96 Islet Capture Microplate is fully compatible with the XF96 Assay Kit sensor cartridge, making all types of XF assays easy to do with islets.

XF Calibrant Solution

(Part #100840-000)

XF Calibrant Solution is optimized for XF sensor cartridge calibration. The calibrant is pre-mixed and ready to use. A 500ml bottle will calibrate 18 sensor cartridges. The calibrant should be stored at 4°C. The calibrant solution can be purchased individually or as part of a FluxPak.

XF Assay Medium

(Part #100965-000)

XF Assay Medium is specially formulated for XF assays. Just add the required amount of glucose and adjust the pH. This ready-made, unbuffered DMEM formulation saves hours of your time. Each Seahorse certified bottle is supplied with the buffer capacity information which will improve your data quality, while lot consistency decreases variability. XF Assay medium is supplied as sterile in 1 liter bottles and packaged two bottles per box.