XFp Extracellular Flux Analyzer

Proven XF Technology, Accessible for Every Lab

Contact Me About XFp

XFp Extracellular Flux Analyzer

XFp Analyzer

XFp Miniplate and cartridge

XFp Miniplate and sensor cartridge

For researchers analyzing precious, limited biomaterial or who just prefer to run a few samples per day, the XFp Analyzer provides powerful XF technology in a streamlined, easy-to-use platform.

The XFp Extracellular Flux Analyzer is built on proven XF technology for monitoring the two major energy pathways of the cell:  Oxidative Phosphorylation, measured by Oxygen Consumption Rate (OCR) and Glycolysis, measured by Extracellular Acidification Rate (ECAR).

Benefits of the new XFp Analyzer:

  • Allows quick assessment of a few samples
  • Runs standard assays without need for special bioenergetics expertise
  • Affordable by individual labs

XFp FluxPaks and XFp Miniplates provide a compact format that can be set up quickly and easily. With six assay wells, the XFp Miniplate has the same well geometry as the XFe96 microplate, making the XFp convenient for pilot experiments and iterative assay design.

The XFp Analyzer easily performs the Gold standard XF assays (metabolic switching, XF Cell Mito Stress Test and XF Glycolysis Stress Test) on small sample sets, making it ideal for:

  • Pairwise comparisons
  • Phenotyping
  • Patient-derived samples

Key Applications for the XFp Analyzer

Seahorse XF technology delivers functional metabolic parameters that have become the gold standard for energy metabolism.  With the XFp Analyzer it is easier than ever to complete your research story with these standard assays:

XF Glycolysis Stress Test
Analyze Metabolic Reprogramming in Primary Immune Cells

XF Glycolysis Stress Test

In order to respond to foreign antigens, immune cells must be able to increase their metabolism.  In these mouse bone-marrow derived macrophages, overnight activation by LPS revved up the glycolytic machinery.  The XFp detected an increase in both glycolysis (ECAR) and glycolytic reserve compared to quiescent cells.

XF Cell Mito Stress Test
Verify impact of gene expression changes on metabolicphenotype

XF Cell Mito Stress Test

Oxidative phosphorylation (OXPHOS) requires the mitochondrial electron transport chain as well as access to substrates such as pyruvate.  Inhibition of mitochondrial pyruvate carrier 1 (MPC1) by UK5099 has no impact on basal OXPHOS but spare respiratory capacity is abrogated in C2C12-derived myotubes. 

XF Phenogram
Detect metabolic switching in a single pairwise experiment

XF Metabolic Switching

Upon induction of certain signaling pathways, cells will shift their mode of energy production.  Activation of HIF-1α by CoCl2 induces a metabolic switch in SKOV-3 cancer cells.  The XF Phenogram shows that OXPHOS (OCR) of the treated cells is reduced and glycolysis (ECAR) is increased relative to the control.

Contact Me About XFp