XFp Extracellular Flux Analyzer

The fastest way to determine the metabolic phenotype of your cells

Contact Me About XFp

XFp Extracellular Flux Analyzer

XFp Analyzer

XFp Miniplate and cartridge

XFp Miniplate and sensor cartridge

For scientists seeking to understand the role of energy metabolism in their disease model, the XFp Extracellular Flux Analyzer provides functional metabolic data to verify gene expression changes or provide a phenotype using only a few thousand live cells.

Using technology backed by over 1500 customer publications, the XFp Analyzer reports the activity of the two major cell energy pathways: Mitochondrial Respiration, measured by Oxygen Consumption Rate (OCR) and Glycolysis, measured by Extracellular Acidification Rate (ECAR).

Benefits of the new XFp Analyzer:

  • Allows quick assessment of BOTH respiration and glycolysis in live cells
  • Runs functional metabolic assays without need for special bioenergetics expertise
  • Generates functional data on as few as 5000 cells per well

The XFp Analyzer, with the patented six-well XFp Miniplate, easily performs gold-standard XF assays (XF Cell Energy Phenotype Test, XF Cell Mito Stress Test and XF Glycolysis Stress Test) on small sample sets, making it ideal for:

  • Pairwise comparisons
  • Phenotyping
  • Patient-derived samples

Key Applications for the XFp Analyzer

Seahorse XF Technology delivers functional metabolic parameters that have become the gold standard for energy metabolism. The XFp Analyzer makes it easier than ever to complete your research with these standard assays:

XF Glycolysis Stress Test
Analyze Metabolic Reprogramming in Primary Immune Cells

XF Glycolysis Stress Test

Immune cells must be able to increase their metabolism in response to foreign antigens. In these mouse bone-marrow derived macrophages, overnight activation by LPS revved up the glycolytic machinery. The XFp detected an increase in both glycolysis (ECAR) and glycolytic reserve compared to quiescent cells.

XF Cell Mito Stress Test
Verify impact of gene expression changes on metabolic phenotype

XF Cell Mito Stress Test

Oxidative phosphorylation (OXPHOS) requires the mitochondrial electron transport chain as well as access to substrates such as pyruvate.  Inhibition of mitochondrial pyruvate carrier 1 (MPC1) by UK5099 has no impact on basal OXPHOS but spare respiratory capacity is abrogated in C2C12-derived myotubes. 

XF Cell Energy Phenotype
Detect metabolic switching in a single pairwise experiment

XF Metabolic Switching

Upon induction of certain signaling pathways, cells will shift their mode of energy production.  Activation of HIF-1α by CoCl2 induces a metabolic switch in SKOV-3 cancer cells.  OCR of the treated cells is reduced and ECAR is increased relative to the control.

Contact Me About XFp