XF Instruments Overview

All XF instruments analyze mitochondrial respiration and glycolysis in live cells, generating data in just minutes. XF Technology uses solid-state sensors to simultaneously measure both oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in every well. All platforms support injection of up to 4 unique compounds per well.

XFp Analyzer
XFp
The XFp Analyzer features fast and easy setup using built-in assay templates and a miniplate format, making it ideal for researchers who run just a few samples per day. Key applications include metabolic phenotyping of rare cell types, patient-derived samples, and other precious cells. The XFp Analyzer is our most affordable XF platform and is suitable for performing our gold standard Stress Tests in a pairwise comparison format.
XFe96 Analyzer
XFe96
The XFe96 Analyzer provides the highest capacity for XF assays at the lowest per-sample cost. The XFe96 enables a large number of experimental groups per assay, making it ideal for labs seeking to maximize design flexibility and sample throughput. Recommended for model organisms, screening applications as well as dose-response studies. Can analyze individual spheroids with the XFe96 Spheroid Micoplate. Validated for assays performed in a hypoxia chamber.
XFe24 Analyzer
XFe24
The XFe24 is validated by hundreds of peer-reviewed publications.

XF Instrument Selection Guide

XFp
Analyzer
XFe96
Analyzer
XFe24
Analyzer
Plate Format Miniplate Microplate Microplate
Number of Assay Wells 6 96 24
Microchamber volume 2 µL 2 µL 7 µL
Assay Working Volume 150-275 µL 150-275 µL 500-1000 µL
Recommended Injection Volume 25 µL 25 µL 75 µL
Controller Integrated full-color touch-screen interface to design basic protocols and run imported templates. Combination computer and touchscreen display with full assay design, control, and analysis capability. Combination computer and touchscreen display with full assay design, control, and analysis capability.
Software Create and run standard assays on controller OR
design on Wave desktop, transfer to Controller, return to desktop for analysis.
Design and analyze assay templates on Controller or Wave desktop. Design and analyze assay templates on Controller or Wave desktop.
Best For
  • Pairwise comparisons
  • Precious biomaterial
  • Patient-derived samples
  • Testing many conditions at one time
  • Dose-response studies
  • Spheroids
  • Model organisms
  • Islets
Key Advantages
  • Easy to set up and run via streamlined interface
  • Standardized protocols for XF Stress Tests
  • Maximum experimental flexibility
  • Highest throughput
  • Validated for hypoxia
  • Lower capital investment

All XF Analyzers Feature:

  • Capacity to perform Gold Standard XF Assays including the Cell Mito Stress Test, Glycolysis Stress Test, and Metabolic Switching
  • Compatibility with both adherent and suspension cells as well as isolated mitochondria.
  • Ability to perform up to 4 independent injections per well with automatic mixing
  • Simultaneous measurement of OCR and ECAR in the same well
  • Sensitivity for small sample sizes
  • Label-free detection in real time
  • Automatic calculation of oxygen consumption rate and extracellular acidification rate
  • Windows-compatible desktop analysis software (Wave) for plotting, reporting, analyzing, and exporting your XF data