Seahorse XF Instruments

Overview

Selection Guide

Cell Lines and Seeding Density

Overview

All Seahorse XF instruments analyze mitochondrial respiration and glycolysis in live cells, generating data in just minutes. Seahorse XF technology uses solid-state sensors to simultaneously measure both oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in every well. All platforms support injection of up to 4 unique compounds per well.

Seahorse XFp Analyzer
XFp
The Seahorse XFp Analyzer features fast and easy setup using built-in assay templates and a miniplate format, making it ideal for researchers who run just a few samples per day. Key applications include metabolic phenotyping, screening patient-derived samples and analysis of rare/precious cells.
Seahorse XFe96 Analyzer
XFe96
The Seahorse XFe96 Analyzer provides the highest capacity for XF assays at the lowest per-sample cost. The Seahorse XFe96 enables a large number of experimental groups per assay, making it ideal for labs seeking to maximize design flexibility and sample throughput. The Seahorse XFe96 Analyzer can analyze individual spheroids with the Seahorse XFe96 Spheroid Microplate. Validated for assays performed in a hypoxia chamber.
Seahorse XFe24 Analyzer
XFe24
The Seahorse XFe24 is validated by hundreds of peer-reviewed publications, and utilizes a larger well format which is suitable for islets and other specialty sample types. Validated for assays performed in a hypoxia chamber.

Selection Guide

Seahorse XFp
Analyzer
Seahorse XFe96
Analyzer
Seahorse XFe24
Analyzer
Plate Format Miniplate Microplate Microplate
Number of Assay Wells 6 96 24
Microchamber volume 2 µL 2 µL 7 µL
Assay Working Volume 150-275 µL 150-275 µL 500-1000 µL
Recommended Injection Volume 25 µL 25 µL 75 µL
Controller Integrated full-color touch-screen interface to design basic protocols and run imported templates. Combination computer and touchscreen display with full assay design, control, and analysis capability. Combination computer and touchscreen display with full assay design, control, and analysis capability.
Software Create and run standard assays on controller OR
design on Wave desktop, transfer to Controller, return to desktop for analysis.
Design and analyze assay templates on Controller or Wave desktop. Design and analyze assay templates on Controller or Wave desktop.
Best For
  • Pairwise comparisons
  • Phenotyping single samples
  • Precious biomaterial
  • Patient-derived samples
  • Assay temperatures lower than 37°C
  • Phenotypic Screening
  • Testing many conditions at one time
  • Dose-response studies
  • Spheroids
  • Islets
  • Larger samples
Key Advantages
  • Easy to set up and run via streamlined interface
  • Standardized protocols for XF Assays
  • Validated for assay temperatures 16-40°C (lower ambient temp required)
  • Maximum experimental flexibility
  • Highest throughput
  • Validated for hypoxia
  • Balances throughput and budget considerations

All Seahorse XF Analyzers Feature:

  • Compatibility with both adherent and suspension cells as well as isolated mitochondria.
  • Ability to perform up to 4 independent injections per well with automatic mixing
  • Automatic calculation of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).
  • Simultaneous measurement of OCR and ECAR in the same well
  • Sensitivity for small sample sizes
  • Label-free detection in real time
  • Windows-compatible desktop analysis software (Wave) for plotting, reporting, analyzing, and exporting your XF data

Cell Lines and Seeding Density

Cell Line Seeding Density Range of Cells/Well
Seahorse XFp/XFe96 Seahorse XFe24
3T3-L1 - Preadipocytes5-7x10310-40x103
A549 Cells4x1042x104
Astrocytes5x103 - 20x103 50x103 - 100x103
BMDM8x10410-50x104
C2C121-2x1042-3x104
Cardiomyocytes (primary)3-5x1044x104 - 15x104
Cortical Neurons (primary)1-4x104 5x104 - 10x104
Dermal Fibroblasts2-4x1041-5x104
H9C21-2x1042-5x104
HCT1161-2x1043-5x104
HEK 2932-5x1045x104
HeLa1-3x1041-5x104
Hepatocytes (primary)1x1041-4x104
HepG21-3x1044-5x104
HUVEC1x1042-3x104
iPSC5x10350-70x103
Isolated mitochondria1-5 µg/well2-10 µg/well
Jurkat1x1051-2x105
MCF10A1-3x1042-4x104
MCF72x1042-4x104
MDA-MB-2311-2x1043-5x104
MEF1x104/1-4x104
PC128x1045x104
RAW 264.78x104 6x104 - 20x104
T-cells (primary)1 - 2x1053x105 - 10x105