XF FluxPaks

FluxPaks simplify your ordering process providing the correct ratio of XF Assay Kits to XF Microplates and XF Calibrant to perform XF assays.

XF96 FluxPaks

For measuring OCR and ECAR
Part #: 101117-001 & 101107-001

Includes 18 XF96 extracellular flux assay kits, 20 XF96 cell culture microplates, and 1 bottle of calibrant solution.

Learn More Buy Now

XF24 FluxPaks

For measuring OCR and ECAR
Part #: 100850-001

Includes 18 XF24 extracellular flux assay kits, 20 XF24 cell culture microplates, and 1 bottle of calibrant solution.

Learn More Buy Now

XF24 Islet FluxPaks

For measuring OCR and ECAR
Part #: 101174-100

Includes 6 XF24 extracellular flux assay kits, 6 XF24 islet capture microplates, and 1 bottle of calibrant solution.

Learn More Buy Now

XF Assay Cartridge

For measuring OCR and ECAR

The XF Assay Cartridge consists of a disposable, 24 or 96 well, dual-analyte sensor cartridge, calibration plate, and lid. The sensor cartridge measures OCR (oxygen consumption rate) and ECAR (extracellular acidification rate).

The two fluorophores for analyte detection are embedded in a polymer spotted on the end of each of the sensor sleeves. The OCR is determined by the amount that oxygen quenches the fluorescence of a fluorescein complex. The ECAR sensor responds to changes in proton concentration [H⁺] as they diffuse across the fluorophore based polymetric substrate. The negative log of proton concentration is equal to the pH. The XF Analyzer's fiber optic wave guide is inserted into the sleeves of the sensor cartridge. Light is pumped through the fiber optic bundles, exciting the fluorophores and the resultant emission is detected and measured.

Transient Microchamber

The sensor cartridge contains barcode information matching it to the corresponding tissue culture plate. The disposable assay kit may be used for hours or days. Sensor cartridges are not able to analyze non matching cell culture plates.

Each sensor cartridge also contains four compound storage and delivery ports per well. A unique multi-channel drug delivery system injects compounds into each cell well on command. This allows multiple measurements to be made using a single population of cells, and increases the effective throughput of the assay plate.

XF Assay Cartidges are supplied in individual containers and are packaged six per box.

XF Assay Cartidges may only be purchased as part of a FluxPak.

FluxPak Consumables: Cost Comparison	XF24	XF96
Assay Microplates per FluxPak	18	18
Consumable Cost per Assay Microplate	$55.89	$55.89
Wells per Assay Microplate	24	96
Cost per Well	$2.33	$0.58
Cost per Injection using all Drug Ports	$0.58	$0.15