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Application Notes, Technical Briefs, & Protocols

Application Notes, Technical Briefs, and Protocols written by cellular bioenergetic scientists for cellular bioenergetic researchers. These articles offer experienced analysis, assay ideas, and reference diagrams to assist you with your research. Can be viewed online or download as a printable PDF.

App Notes

    (Summary of 3 to 4 publications utilizing XF technology in an application area)

• Functional validation of Genomic and Proteomic data
• Assessing mitochondrial dysfunction in primary cardiomyocytes
• Bioenergetic Analysis of Primary Neuronal Deficiencies
• Bioenergetic analysis of suspension cells: hematopoietic stem cells and lymphocytes
• Defining metabolic phenotypes in cancer cells
• Direct Comparison of Radiometric versus XF24 Flux Assays to Measure Substrate Utilization
• Measuring bioenergetic profiles of human adipocytes
• Measuring fatty acid oxidation in muscle cells
• Measuring glycolysis and oxidative metabolism in cancer cells
• Metabolic Pathway Determination Using the XF24 Analyzer
• Screening for Mitochondrial Biogenesis and Toxicity
• Understanding the Relationship Between Bioenergetic Rates and ATP Turnover

Tech Briefs

    (Guidelines to modify standard XF assays for different samples)

• Analyzing microgram quantities of isolated mitochondria in the XF24 Analyzer
• Measuring mitochondrial respiration in intact skeletal muscle fibers

Protocols

    (Step-by-step instructions to prepare and run standard XF assays)

• Simultaneous measurement of the TCA cycle and respiration in isolated mitochondria with the XF24-3 Analyzer and CO₂ sensor cartridge
• Simultaneous measurement of the TCA cycle and respiration in cells with the XF24-3 Analyzer and CO₂ sensor cartridge
• Preparation of Bovine Serum Albumin (BSA)-Conjugated Palmitate
• Islet Assay using the XF24 Islet Capture Microplate

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