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The power of real-time kinetics unlocks essential cellular bioenergetic data

The Seahorse Extracellular Flux (XF) Analyzer, and XF Stress Test Kits, make cellular bioenergetic studies simple, efficient and user-friendly. The first new in vitro metabolic measurement in 50 years, XF Analyzers non-invasively profile the metabolic activity of cells in minutes, offering scientists a physiologic cell based assay for determination of basal oxygen consumption, glycolysis rates, ATP turnover and respiratory capacity in a single experiment to assess mitochondrial dysfunction.

Discover why over 1200 scientists are using the XF Analyzer for a faster, better and more accurate measurement of real-time cellular bioenergetics.

By measuring the two major energy producing pathways of the cell simultaneously, mitochondrial respiration and glycolysis, scientists get the most physiologically relevant bioenergetic assay available, resulting in a better overall view of metabolism. The XF Analyzer also measures fatty acid oxidation and metabolism of glucose and amino acids for kinetic metabolic information.

No labels, radioactivity, dyes, trypsin, or permeabilization!

Adherent cells, primary cells, suspension cells and isolated mitochondria can be analyzed easily. XF Analyzers allow you to measure mitochondrial function and dysfunction in living cells with less setup and clean up than Clark electrode methods.

XF Cell Mito Stress Test Kit Profile

The key parameters of mitochondrial functions: basal respiration (1), ATP production (2), proton leak (3), and maximal respiration (4) or spare respiratory capacity (5).

Measure basal respiration, ATP turnover, proton leak, and spare respiratory capacity.

Each XF Cell Mito Stress Test Kit eliminates the questions and concerns of where to source the reagents, what concentrations are optimal, and best storage conditions. Each convenient kit contains enough reagents to run six XF24 or XF96 microplates, allowing you to run a full mitochondrial profile in each well. With the reagents now ready at your fingertips, the XF Cell Mito Stress Test Kit is your ultimate tool for assay optimization.

Everything you need to measure mitochondrial (dys)function in cells