Applications in Translational Medicine

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App Notes & Tech Briefs

Bioenergetic analysis of suspension cells: hematopoietic stem cells and lymphocytes

A real time assay that quantifies the ATP and biosynthetic demands of immune cell proliferation, differentiation and effector function.

Normal suspension cells, such as hematopoietic cells and lymphocytes are responsible for supplying oxygen to the body and protecting the host. Cellular metabolism is markedly dynamic in immune responses during proliferation and immune effector functions...
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Publications

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Mechanisms underlying metabolic and neural defects in zebrafish and human multiple acyl-CoA dehydrogenase deficiency (MADD)
Song Y, Selak M A, Watson C T, Coutts C, Scherer P C, Panzer J A, Gibbs S, Scott M O, Willer G, Gregg R G, Ali D W, Bennett M J and Balice-Gordon R J
PLoS One; 2009 Dec 17. 4 (12):e8329.
Regulation of skeletal muscle oxidative capacity and insulin signaling by the mitochondrial rhomboid protease PARL
Civitarese A E, MacLean P S, Carling S, Kerr-Bayles L, McMillan R P, Pierce A, Becker T C, Moro C, Finlayson J, Lefort N, Newgard C B, Mandarino L, Cefalu W, Walder K, Collier G R, Hulver M W, Smith S R and Ravussin E
Cell Metab; 2010 May 5. 11 (5):412-426.
Medroxyprogesterone Acetate Antagonizes Estrogen Up-Regulation of Brain Mitochondrial Function
Irwin R W, Yao J, Ahmed S S, Hamilton R T, Cadenas E and Brinton R D
Endocrinology; 2010 Dec 15. 152 (2):556-567.
Acquisition of temozolomide chemoresistance in gliomas leads to remodeling of mitochondrial electron transport chain
Oliva C R, Nozell S E, Diers A, McClugage S G, 3rd, Sarkaria J N, Markert J M, Darley-Usmar V M, Bailey S M, Gillespie G Y, Landar A and Griguer C E
J Biol Chem; 2010 Dec 17. 285 (51):39759-39767.
Apolipoprotein e4 domain interaction mediates detrimental effects on mitochondria and is a potential therapeutic target for Alzheimer disease
Chen H K, Ji Z S, Dodson S E, Miranda R D, Rosenblum C I, Reynolds I J, Freedman S B, Weisgraber K H, Huang Y and Mahley R W
J Biol Chem; 2011 Feb 18. 286 (7):5215-5221.
Exploring a glycolytic inhibitor for the treatment of an FH-deficient type-2 papillary RCC
Yamasaki T, Tran T A, Oz O K, Raj G V, Schwarz R E, Deberardinis R J, Zhang X and Brugarolas J
Nat Rev Urol; 2011 Mar 1. 8 (3):165-171.
Mutation in the mitochondrial tRNAVal causes mitochondrial encephalopathy, lactic acidosis and stroke-like episodes
Glatz C, D'Aco K, Smith S and Sondheimer N
Mitochondrion; 2011 Jul 1. 11 (4):615-619.
2-deoxy-d-glucose treatment induces ketogenesis, sustains mitochondrial function, and reduces pathology in female mouse model of Alzheimer's disease
Yao J, Chen S, Mao Z, Cadenas E and Brinton R D
PLoS One. 2011 Jul 1; [Epub ahead of print].
Targeting GLUT1 and the Warburg Effect in Renal Cell Carcinoma by Chemical Synthetic Lethality
Chan D A, Sutphin P D, Nguyen P, Turcotte S, Lai E W, Banh A, Reynolds G E, Chi J T, Wu J, Solow-Cordero D E, Bonnet M, Flanagan J U, Bouley D M, Graves E E, Denny W A, Hay M P and Giaccia A J
Sci Transl Med; 2011 Aug 3. 3 (94):94ra70.
Withaferin a-induced apoptosis in human breast cancer cells is mediated by reactive oxygen species
Hahm E R, Moura M B, Kelley E E, Van Houten B, Shiva S and Singh S V
PLoS One. 2011 Aug 10; [Epub ahead of print].
Hydrogen sulfide replacement therapy protects the vascular endothelium in hyperglycemia by preserving mitochondrial function
Suzuki K, Olah G, Modis K, Coletta C, Kulp G, Gero D, Szoleczky P, Chang T, Zhou Z, Wu L, Wang R, Papapetropoulos A and Szabo C
Proc Natl Acad Sci U S A; 2011 Aug 16. 108 (33):13829-13834.
SRT1720 improves survival and healthspan of obese mice
Minor R K, Baur J A, Gomes A P, Ward T M, Csiszar A, Mercken E M, Abdelmohsen K, Shin Y-K, Canto C, Scheibye-Knudsen M, Krawczyk M, Irusta P M, Martin-Montalvo A, Hubbard B P, Zhang Y, Lehrmann E, White A A, Price N L, Swindell W R, Pearson K J, Becker K G, Bohr V A, Gorospe M, Egan J M, Talan M I, Auwerx J, Westphal C H, Ellis J L, Ungvari Z, Vlasuk G P, Elliott P J, Sinclair D A and de Cabo R
Sci. Rep.; 2011 Aug 18. 1 (2011)
Human iPSCs Harbor Homoplasmic and Heteroplasmic Mitochondrial DNA Mutations While Maintaining hESC-Like Metabolic Reprogramming
Prigione A, Lichtner B, Kuhl H, Struys E A, Wamelink M, Lehrach H, Ralser M, Timmermann B and Adjaye J
Stem Cells; 2011 Sep 1. 29 (9):1338-1348.
Platelet Mitochondrial Dysfunction is Evident in Type 2 Diabetes in Association with Modifications of Mitochondrial Anti-Oxidant Stress Proteins
Avila C, Huang R J, Stevens M V, Aponte A M, Tripodi D, Kim K Y and Sack M N
Exp Clin Endocrinol Diabetes. 2011 Sep 15; [Epub ahead of print].
The Transcriptional Coactivators, PGC-1? and ?, Cooperate to Maintain Cardiac Mitochondrial Function During the Early Stages of Insulin Resistance
Mitra R, Nogee D P, Zechner J F, Yea K, Gierasch C M, Kovacs A, Medeiros D M, Kelly D P and Duncan J G
J Mol Cell Cardiol. 2011 Oct 20; [Epub ahead of print].
The effects of idebenone on mitochondrial bioenergetics
Giorgio V, Petronilli V, Ghelli A, Carelli V, Rugolo M, Lenaz G and Bernardi P
Biochimica et Biophysica Acta (BBA) - Bioenergetics; 2011 Nov 4.
Galactose enhances oxidative metabolism and reveals mitochondrial dysfunction in human primary muscle cells
Aguer C, Gambarotta D, Mailloux R J, Moffat C, Dent R, McPherson R and Harper M E
PLoS One. 2011 Dec 15; [Epub ahead of print].
Identification and analysis of the active phytochemicals from the anti-cancer botanical extract bezielle
Chen V, Staub R E, Baggett S, Chimmani R, Tagliaferri M, Cohen I and Shtivelman E
PLoS One. 2012 Jan 17; [Epub ahead of print].
Microscale oxygraphy reveals OXPHOS impairment in MRC mutant cells
Invernizzi F, D'Amato I, Jensen P B, Ravaglia S, Zeviani M and Tiranti V
Mitochondrion. 2012 Jan 28; [Epub ahead of print].

Technical Posters

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Webinars

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Mitochondrial Bioenergetics in Disease: Toward a Mitochondrial Therapy of Muscular Dystrophies and Beyond
Paolo Bernardi, MD, University of Padova
September 15, 2009
Acute Brain Injury: Assessing Mitochondrial Dysfunction and the Role of Calpain Proteases in vitro
Brian Polster, PhD, University of Maryland Medical School
August 19, 2009
How to Measure Mitochondrial Function in Whole Islets. Sensing the nutrient sensor: measuring bioenergetic parameters from intact mouse and human islets of langerhans
Orian Shirihai, MD, PhD, Boston University Medical Center
July 16, 2009
Primary Renal Proximal Tubules as a Platform for Mitochondrial Biogenesis and Toxicity Assays
Craig Beeson, PhD , Medical University of South Carolina
June 24, 2009
Cybrid Modeling of Sporadic Neurodegenerative Disorders
Russell H. Swerdlow, MD, University of Kansas School of Medicine
March 16, 2009
Bioenergetic Profiling in Translational Neuroscience
Jon Nilsen, PhD, University of Southern California
June 25, 2008
Human Disorders of Bioenergetics
Vamsi Mootha, MD, Harvard Medical School Center for Human Genetics, Massachusetts General Hospital
February 26, 2008

Translational medicine is an emerging field within medical research which seeks to draw inferences from research in basic biomedical sciences such as molecular and cell biology that are of significance for patient care. With its focus on removing barriers to multi-disciplinary collaboration, translational research has the potential to drive the advancement of applied science.

An attempt to bridge these barriers has been undertaken particularly in the medical domain where the term translational medicine has been applied to a research approach that seeks to move "from bench to bedside" or from laboratory experiments through clinical trials to actual point-of-care patient applications.

Enabling a greater understanding of cellular bioenergetics, the XF Analyzer allows the measurement of cellular metabolism in real time, in a microplate. By measuring the oxygen consumption rate (OCR)—a measure of mitochondrial respiration; as well as the extracellular acidification rate (ECAR)—a measure of glycolysis, you will be provided with the most physiologically relevant in vitro measurement for your bioenergetic studies.