Applications in Obesity, Diabetes, & Metabolic Disorders

App Notes & Tech Briefs

• Measuring bioenergetic profiles of human adipocytes

  Measuring white adipose tissue (WAT) mitochondrial function reveals impaired fatty acid stimulated uncoupling from obese individuals

  White adipose tissue (WAT) is increasingly being recognized for its importance in regulating metabolic homeostasis, in addition to passive lipid storage. However, mature adipocytes and WAT are challenging to study because they are laden with lipid and float on top of media...

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• Measuring mitochondrial respiration in intact skeletal muscle fibers

  Assessment of mitochondrial function in skeletal muscle is a powerful tool for modeling neuromuscular conditions including muscular dystrophy, aging and cachexia. Similarly, skeletal muscle plays an important role in normal and pathological metabolism, such as obesity and diabetes. The majority of investigations use measurements of single mitochondrial enzymes as a surrogate to measuring mitochondrial function which does not provide a complete physiological readout of the mitochondria...

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• Measuring fatty acid oxidation in muscle cells

  A sensitive non-radiometric real-time assay of FAO

  Altered fatty acid metabolism by skeletal muscle can lead to insulin resistance and the accumulation of lipid in non-adipose tissue. This accumulation plays a critical role in the pathogenesis of chronic diseases including diabetes and heart failure...

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• Direct Comparison of Radiometric versus XF24 Flux Assays to Measure Substrate Utilization

  With accuracies comparable to those obtained with radiometric assays, the Seahorse XF24 Analyzer measures substrate utilization via extracellular fluxes of metabolites such as oxygen and protons to estimate metabolic fluxes due to mitochondrial respiration and glycolysis, respectively...

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• Analyzing microgram quantities of isolated mitochondria in the XF24 Analyzer

  Enhanced appreciation of the role of altered mitochondrial function in tumorigenesis, aging, neurodegenerative, metabolic, and cardiovascular diseases has stimulated the development of a variety of new approaches for assessing mitochondrial function and dysfunction. As the field moves towards the discovery of mitochondrial-related, molecular mechanisms that underlie disease, and drugs to prevent or reverse disease development, the demand for higher throughput and more sensitive assay methods increases...

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Publications

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• Bile acids induce energy expenditure by promoting intracellular thyroid hormone activation

  Watanabe M, Houten S M, Mataki C, Christoffolete M A, Kim B W, Sato H, Messaddeq N, Harney J W, Ezaki O, Kodama T, Schoonjans K, Bianco A C and Auwerx J

  Nature; 2006 Jan 26. 439 (7075):484-489.

• The small polyphenolic molecule kaempferol increases cellular energy expenditure and thyroid hormone activation

  da-Silva W S, Harney J W, Kim B W, Li J, Bianco S D, Crescenzi A, Christoffolete M A, Huang S A and Bianco A C

  Diabetes; 2007 Mar 1. 56 (3):767-776.

• Peroxisome proliferator activator receptor gamma coactivator-1 expression is reduced in obesity: potential pathogenic role of saturated fatty acids and p38 mitogen-activated protein kinase activation

  Crunkhorn S, Dearie F, Mantzoros C, Gami H, da Silva W S, Espinoza D, Faucette R, Barry K, Bianco A C and Patti M E

  J Biol Chem; 2007 May 25. 282 (21):15439-15450.

• Fission and selective fusion govern mitochondrial segregation and elimination by autophagy

  Twig G, Elorza A, Molina A J, Mohamed H, Wikstrom J D, Walzer G, Stiles L, Haigh S E, Katz S, Las G, Alroy J, Wu M, Py B F, Yuan J, Deeney J T, Corkey B E and Shirihai O S

  EMBO J; 2008 Jan 23. 27 (2):433-446.

• Specific SIRT1 activation mimics low energy levels and protects against diet-induced metabolic disorders by enhancing fat oxidation

  Feige J N, Lagouge M, Canto C, Strehle A, Houten S M, Milne J C, Lambert P D, Mataki C, Elliott P J and Auwerx J

  Cell Metab; 2008 Nov 1. 8 (5):347-358.

• AMPK regulates energy expenditure by modulating NAD+ metabolism and SIRT1 activity

  Canto C, Gerhart-Hines Z, Feige J N, Lagouge M, Noriega L, Milne J C, Elliott P J, Puigserver P and Auwerx J

  Nature; 2009 Apr 23. 458 (7241):1056-1060.

• TGR5-mediated bile acid sensing controls glucose homeostasis

  Thomas C, Gioiello A, Noriega L, Strehle A, Oury J, Rizzo G, Macchiarulo A, Yamamoto H, Mataki C, Pruzanski M, Pellicciari R, Auwerx J and Schoonjans K

  Cell Metab; 2009 Sep 1. 10 (3):167-177.

• C/EBPalpha and the corepressors CtBP1 and CtBP2 regulate repression of select visceral white adipose genes during induction of the brown phenotype in white adipocytes by peroxisome proliferator-activated receptor gamma agonists

  Vernochet C, Peres S B, Davis K E, McDonald M E, Qiang L, Wang H, Scherer P E and Farmer S R

  Mol Cell Biol; 2009 Sep 1. 29 (17):4714-4728.

• KSR2 is an essential regulator of AMP kinase, energy expenditure, and insulin sensitivity

  Costanzo-Garvey D L, Pfluger P T, Dougherty M K, Stock J L, Boehm M, Chaika O, Fernandez M R, Fisher K, Kortum R L, Hong E G, Jun J Y, Ko H J, Schreiner A, Volle D J, Treece T, Swift A L, Winer M, Chen D, Wu M, Leon L R, Shaw A S, McNeish J, Kim J K, Morrison D K, Tschop M H and Lewis R E

  Cell Metab; 2009 Nov 1. 10 (5):366-378.

• Mice lacking Pctp /StarD2 exhibit increased adaptive thermogenesis and enlarged mitochondria in brown adipose tissue

  Kang H W, Ribich S, Kim B W, Hagen S J, Bianco A C and Cohen D E

  J Lipid Res; 2009 Nov 1. 50 (11):2212-2221.

• Tight coupling between glucose and mitochondrial metabolism in clonal beta-cells is required for robust insulin secretion

  Malmgren S, Nicholls D G, Taneera J, Bacos K, Koeck T, Tamaddon A, Wibom R, Groop L, Ling C, Mulder H and Sharoyko V V

  J Biol Chem; 2009 Nov 20. 284 (47):32395-32404.

• Insulin signaling regulates mitochondrial function in pancreatic beta-cells

  Liu S, Okada T, Assmann A, Soto J, Liew C W, Bugger H, Shirihai O S, Abel E D and Kulkarni R N

  PLoS One; 2009 Nov 24. 4 (11):e7983.

• Insulin resistance and altered systemic glucose metabolism in mice lacking Nur77

  Chao L C, Wroblewski K, Zhang Z, Pei L, Vergnes L, Ilkayeva O R, Ding S Y, Reue K, Watt M J, Newgard C B, Pilch P F, Hevener A L and Tontonoz P

  Diabetes; 2009 Dec 1. 58 (12):2788-2796.

• The histone deacetylase Sirt6 regulates glucose homeostasis via Hif1α

  Zhong L, D'Urso A, Toiber D, Sebastian C, Henry R E, Vadysirisack D D, Guimaraes A, Marinelli B, Wikstrom J D, Nir T, Clish C B, Vaitheesvaran B, Iliopoulos O, Kurland I, Dor Y, Weissleder R, Shirihai O S, Ellisen L W, Espinosa J M and Mostoslavsky R

  Cell; 2010 Jan 22. 140 (2):280-293.

• A plasma signature of human mitochondrial disease revealed through metabolic profiling of spent media from cultured muscle cells

  Shaham O, Slate N G, Goldberger O, Xu Q, Ramanathan A, Souza A L, Clish C B, Sims K B and Mootha V K

  Proc Natl Acad Sci U S A; 2010 Jan 26. 107 (4):1571-1575.

• Nutrient-sensitized screening for drugs that shift energy metabolism from mitochondrial respiration to glycolysis

  Gohil V M, Sheth S A, Nilsson R, Wojtovich A P, Lee J H, Perocchi F, Chen W, Clish C B, Ayata C, Brookes P S and Mootha V K

  Nat Biotechnol; 2010 Mar 1. 28 (3):249-255.

• Interdependence of AMPK and SIRT1 for metabolic adaptation to fasting and exercise in skeletal muscle

  Canto C, Jiang L Q, Deshmukh A S, Mataki C, Coste A, Lagouge M, Zierath J R and Auwerx J

  Cell Metab; 2010 Mar 3. 11 (3):213-219.

• The homeobox protein Prox1 is a negative modulator of ERR{α}/PGC-1{α} bioenergetic functions

  Charest-Marcotte A, Dufour C R, Wilson B J, Tremblay A M, Eichner L J, Arlow D H, Mootha V K and Giguere V

  Genes Dev; 2010 Mar 15. 24 (6):537-542.

• Mammalian target of rapamycin complex 1 suppresses lipolysis, stimulates lipogenesis, and promotes fat storage

  Chakrabarti P, English T, Shi J, Smas C M and Kandror K V

  Diabetes; 2010 Apr 1. 59 (4):775-781.

• Regulation of skeletal muscle oxidative capacity and insulin signaling by the mitochondrial rhomboid protease PARL

  Civitarese A E, MacLean P S, Carling S, Kerr-Bayles L, McMillan R P, Pierce A, Becker T C, Moro C, Finlayson J, Lefort N, Newgard C B, Mandarino L, Cefalu W, Walder K, Collier G R, Hulver M W, Smith S R and Ravussin E

  Cell Metab; 2010 May 5. 11 (5):412-426.

• Pyruvate dehydrogenase kinase 1 controls mitochondrial metabolism and insulin secretion in INS-1 832/13 clonal beta-cells

  Krus U, Kotova O, Spegel P, Hallgard E, Sharoyko V V, Vedin A, Moritz T, Sugden M C, Koeck T and Mulder H

  Biochem J; 2010 Jul 1. 429 (1):205-213.

• Glucose regulates enzymatic sources of mitochondrial NADPH in skeletal muscle cells, a novel role for glucose-6-phosphate dehydrogenase

  Mailloux R J and Harper M E

  FASEB J; 2010 Jul 1. 24 (7):2495-2506.

• High glucose disrupts mitochondrial morphology in retinal endothelial cells: implications for diabetic retinopathy

  Trudeau K, Molina A J, Guo W and Roy S

  Am J Pathol; 2010 Jul 1. 177 (1):447-455.

• Adipose acyl-CoA synthetase-1 directs fatty acids toward beta-oxidation and is required for cold thermogenesis

  Ellis J M, Li L O, Wu P C, Koves T R, Ilkayeva O, Stevens R D, Watkins S M, Muoio D M and Coleman R A

  Cell Metab; 2010 Jul 4. 12 (1):53-64.

• Fibroblast growth factor 21 regulates energy metabolism by activating the AMPK-SIRT1-PGC-1α pathway

  Chau M D, Gao J, Yang Q, Wu Z and Gromada J

  Proc Natl Acad Sci U S A; 2010 Jul 13. 107 (28):12553-12558.

• Wnt signaling regulates mitochondrial physiology and insulin sensitivity

  Yoon J C, Ng A, Kim B H, Bianco A, Xavier R J and Elledge S J

  Genes Dev; 2010 Jul 15. 24 (14):1507-1518.

• A model of Costeff Syndrome reveals metabolic and protective functions of mitochondrial OPA3

  Pei W, Kratz L E, Bernardini I, Sood R, Yokogawa T, Dorward H, Ciccone C, Kelley R I, Anikster Y, Burgess H A, Huizing M and Feldman B

  Development; 2010 Aug 1. 137 (15):2587-2596.

• Pyruvate suppresses PGC1α expression and substrate utilization despite increased respiratory chain content in C2C12 myotubes

  Philp A, Perez-Schindler J, Green C, Hamilton D L and Baar K

  Am J Physiol Cell Physiol; 2010 Aug 1. 299 (2):C240-250.

• Respiration in adipocytes is inhibited by reactive oxygen species

  Wang T, Si Y, Shirihai O S, Si H, Schultz V, Corkey R F, Hu L, Deeney J T, Guo W and Corkey B E

  Obesity (Silver Spring); 2010 Aug 1. 18 (8):1493-1502.

• Cardiolipin remodeling by ALCAT1 links oxidative stress and mitochondrial dysfunction to obesity

  Li J, Romestaing C, Han X, Li Y, Hao X, Wu Y, Sun C, Liu X, Jefferson L S, Xiong J, Lanoue K F, Chang Z, Lynch C J, Wang H and Shi Y

  Cell Metab; 2010 Aug 4. 12 (2):154-165.

• A high-throughput respirometric assay for mitochondrial biogenesis and toxicity

  Beeson C C, Beeson G C and Schnellmann R G

  Anal Biochem; 2010 Sep 1. 404 (1):75-81.

• Absence of thyroid hormone activation during development underlies a permanent defect in adaptive thermogenesis

  Hall J A, Ribich S, Christoffolete M A, Simovic G, Correa-Medina M, Patti M E and Bianco A C

  Endocrinology; 2010 Sep 1. 151 (9):4573-4582.

• Acute stimulation of white adipocyte respiration by PKA-induced lipolysis

  Yehuda-Shnaidman E, Buehrer B, Pi J, Kumar N and Collins S

  Diabetes; 2010 Oct 1. 59 (10):2474-2483.

• SIRT4 regulates fatty acid oxidation and mitochondrial gene expression in liver and muscle cells

  Nasrin N, Wu X, Fortier E, Feng Y, Bare O C, Chen S, Ren X, Wu Z, Streeper R S and Bordone L

  J Biol Chem; 2010 Oct 15. 285 (42):31995-32002.

• Mitochondrial matrix pH controls oxidative phosphorylation and metabolism-secretion coupling in INS-1E clonal beta cells

  Akhmedov D, Braun M, Mataki C, Park K S, Pozzan T, Schoonjans K, Rorsman P, Wollheim C B and Wiederkehr A

  FASEB J; 2010 Nov 1. 24 (11):4613-4626.

• Heart-type fatty acid binding protein is essential for efficient brown adipose tissue fatty acid oxidation and cold tolerance

  Vergnes L, Chin R, Young S G and Reue K

  J Biol Chem; 2010 Nov 1. 286 (1):380-390.

• 9-oxo-10(E),12(E)-octadecadienoic acid derived from tomato is a potent PPAR α agonist to decrease triglyceride accumulation in mouse primary hepatocytes

  Kim Y I, Hirai S, Takahashi H, Goto T, Ohyane C, Tsugane T, Konishi C, Fujii T, Inai S, Iijima Y, Aoki K, Shibata D, Takahashi N and Kawada T

  Mol Nutr Food Res. 2010 Nov 18; [Epub ahead of print].

• Mechanisms of metformin action on glucose transport and metabolism in human adipocytes

  Grisouard J, Timper K, Radimerski T M, Frey D M, Peterli R, Kola B, Korbonits M, Herrmann P, Krahenbuhl S, Zulewski H, Keller U, Muller B and Christ-Crain M

  Biochem Pharmacol; 2010 Dec 1. 80 (11):1736-1745.

• Identification of inducible brown adipocyte progenitors residing in skeletal muscle and white fat

  Schulz T J, Huang T L, Tran T T, Zhang H, Townsend K L, Shadrach J L, Cerletti M, McDougall L E, Giorgadze N, Tchkonia T, Schrier D, Falb D, Kirkland J L, Wagers A J and Tseng Y H

  Proc Natl Acad Sci U S A; 2011 Jan 4. 108 (1):143-148.

• A common variant in TFB1M is associated with reduced insulin secretion and increased future risk of type 2 diabetes

  Koeck T, Olsson A H, Nitert M D, Sharoyko V V, Ladenvall C, Kotova O, Reiling E, Ronn T, Parikh H, Taneera J, Eriksson J G, Metodiev M D, Larsson N G, Balhuizen A, Luthman H, Stancakova A, Kuusisto J, Laakso M, Poulsen P, Vaag A, Groop L, Lyssenko V, Mulder H and Ling C

  Cell Metab; 2011 Jan 5. 13 (1):80-91.

• The adipocyte-expressed forkhead transcription factor foxc2 regulates metabolism through altered mitochondrial function

  Lidell M E, Seifert E L, Westergren R, Heglind M, Gowing A, Sukonina V, Arani Z, Itkonen P, Wallin S, Westberg F, Fernandez-Rodriguez J, Laakso M, Nilsson T, Peng X R, Harper M E and Enerback S

  Diabetes; 2011 Feb 1. 60 (2):427-435.

• Wnt signaling regulates hepatic metabolism

  Liu H, Fergusson M M, Wu J J, Rovira, II, Liu J, Gavrilova O, Lu T, Bao J, Han D, Sack M N and Finkel T

  Sci Signal; 2011 Feb 1. 4 (158):ra6.

• The chemical chaperones tauroursodeoxycholic and 4-phenylbutyric acid accelerate thyroid hormone activation and energy expenditure

  da-Silva W S, Ribich S, Drigo R A, Castillo M, Patti M E and Bianco A C

  FEBS Lett; 2011 Feb 4. 585 (3):539-544.

• Telomere dysfunction induces metabolic and mitochondrial compromise

  Sahin E, Colla S, Liesa M, Moslehi J, Muller F L, Guo M, Cooper M, Kotton D, Fabian A J, Walkey C, Maser R S, Tonon G, Foerster F, Xiong R, Wang Y A, Shukla S A, Jaskelioff M, Martin E S, Heffernan T P, Protopopov A, Ivanova E, Mahoney J E, Kost-Alimova M, Perry S R, Bronson R, Liao R, Mulligan R, Shirihai O S, Chin L and DePinho R A

  Nature; 2011 Feb 9. 470 (7334):359-365.

• Mesodermal developmental gene Tbx15 impairs adipocyte differentiation and mitochondrial respiration

  Gesta S, Bezy O, Mori M A, Macotela Y, Lee K Y and Kahn C R

  Proc Natl Acad Sci U S A; 2011 Feb 15. 108 (7):2771-2776.

• Corepressor SMRT promotes oxidative phosphorylation in adipose tissue and protects against diet-induced obesity and insulin resistance

  Fang S, Suh J M, Atkins A R, Hong S H, Leblanc M, Nofsinger R R, Yu R T, Downes M and Evans R M

  Proc Natl Acad Sci U S A; 2011 Feb 22. 108 (8):3412-3417.

• Uncoupling protein-2 attenuates glucose-stimulated insulin secretion in INS-1E insulinoma cells by lowering mitochondrial reactive oxygen species

  Affourtit C, Jastroch M and Brand M D

  Free Radic Biol Med; 2011 Mar 1. 50 (5):609-616.

• Dietary fat sensing via fatty acid oxidation in enterocytes: possible role in the control of eating

  Langhans W, Leitner C and Arnold M

  Am J Physiol Regul Integr Comp Physiol; 2011 Mar 1. 300 (3):R554-565.

• Inactivation of the mitochondrial carrier SLC25A25 (ATP-MG2+/Pi transporter) reduces physical endurance and metabolic efficiency in mice

  Anunciado-Koza R P, Zhang J, Ukropec J, Bajpeyi S, Koza R A, Rogers R C, Cefalu W T, Mynatt R L and Kozak L P

  J Biol Chem; 2011 Apr 1. 286 (13):11659-11671.

• Metabolomic analyses reveal profound differences in glycolytic and tricarboxylic acid cycle metabolism in glucose-responsive and -unresponsive clonal beta-cell lines

  Spegel P, Malmgren S, Sharoyko V V, Newsholme P, Koeck T and Mulder H

  Biochem J; 2011 Apr 1. 435 (1):277-284.

• Ncb5or deficiency increases fatty acid catabolism and oxidative stress

  Xu M, Wang W, Frontera J R, Neely M C, Lu J J, Aires D, Hsu F F, Turk J, Swerdlow R H, Carlson S E and Zhu H

  J Biol Chem; 2011 Apr 1. 286 (13):11141-11154.

• PARP-2 Regulates SIRT1 Expression and Whole-Body Energy Expenditure

  Bai P, Canto C, Brunyanszki A, Huber A, Szanto M, Cen Y, Yamamoto H, Houten S M, Kiss B, Oudart H, Gergely P, Menissier-de Murcia J, Schreiber V, Sauve A A and Auwerx J

  Cell Metab; 2011 Apr 6. 13 (4):450-460.

• PARP-1 Inhibition Increases Mitochondrial Metabolism through SIRT1 Activation

  Bai P, Canto C, Oudart H, Brunyanszki A, Cen Y, Thomas C, Yamamoto H, Huber A, Kiss B, Houtkooper R H, Schoonjans K, Schreiber V, Sauve A A, Menissier-de Murcia J and Auwerx J

  Cell Metab; 2011 Apr 6. 13 (4):461-468.

• Activation of peroxisome proliferator-activated receptor-alpha enhances fatty acid oxidation in human adipocytes

  Lee J Y, Hashizaki H, Goto T, Sakamoto T, Takahashi N and Kawada T

  Biochem Biophys Res Commun; 2011 Apr 22. 407 (4):818-822.

• To eat or not to eat: neuronal metabolism, mitophagy, and Parkinson's disease

  Gusdon A M and Chu C T

  Antioxid Redox Signal; 2011 May 15. 14 (10):1979-1987.

• Reduced cytochrome C is an essential regulator of sustained insulin secretion by pancreatic islets

  Jung S R, Kuok I T, Couron D, Rizzo N, Margineantu D H, Hockenbery D M, Kim F and Sweet I R

  J Biol Chem; 2011 May 20. 286 (20):17422-17434.

• High fat diet induces an initial adaptation of mitochondrial bioenergetics in the kidney despite evident oxidative stress and mitochondrial ROS production

  Ruggiero C, Ehrenshaft M, Cleland E and Stadler K

  Am J Physiol Endocrinol Metab; 2011 Jun 1. 300 (6):1047-1058.

• Glutathionylation acts as a control switch for uncoupling proteins UCP2 and UCP3

  Mailloux R J, Seifert E L, Bouillaud F, Aguer C, Collins S and Harper M E

  J Biol Chem; 2011 Jun 17. 286 (24):21865-21875.

• Activation of peroxisome proliferator-activated receptor-[alpha] (PPAR[alpha]) suppresses postprandial lipidemia through fatty acid oxidation in enterocytes

  Kimura R, Takahashi N, Murota K, Yamada Y, Niiya S, Kanzaki N, Murakami Y, Moriyama T, Goto T and Kawada T

  Biochemical and Biophysical Research Communications; 2011 Jun 24. 410 (1):40549.

• Mice lacking microRNA 133a develop dynamin 2–dependent centronuclear myopathy

  Liu N, Bezprozvannaya S, Shelton J M, Frisard M I, Hulver M W, McMillan R P, Wu Y, Voelker K A, Grange R W, Richardson J A, Bassel-Duby R and Olson E N

  The Journal of Clinical Investigation; 2011 Jul 1. 121 (8):3258-3268.

• The dynamin-related GTPase Opa1 is required for glucose-stimulated ATP production in pancreatic beta cells

  Zhang Z, Wakabayashi N, Wakabayashi J, Tamura Y, Song W J, Sereda S, Clerc P, Polster B M, Aja S M, Pletnikov M V, Kensler T W, Shirihai O S, Iijima M, Hussain M A and Sesaki H

  Mol Biol Cell; 2011 Jul 1. 22 (13):2235-2245.

• Atrial natriuretic peptide regulates lipid mobilization and oxygen consumption in human adipocytes by activating AMPK

  Souza S C, Chau M D, Yang Q, Gauthier M S, Clairmont K B, Wu Z, Gromada J and Dole W P

  Biochem Biophys Res Commun; 2011 Jul 8. 410 (3):398-403.

• Differential regulation of metabolism by nitric oxide and S-nitrosothiols in endothelial cells

  Diers A R, Broniowska K A, Darley-Usmar V M and Hogg N

  Am J Physiol Heart Circ Physiol; 2011 Sep 1. 301 (3):H803-812.

• A Peroxisome Proliferator-activated Receptor {gamma} (PPAR{gamma})/PPAR{gamma} Coactivator 1{beta} Autoregulatory Loop in Adipocyte Mitochondrial Function

  Deng T, Sieglaff D H, Zhang A, Lyon C J, Ayers S D, Cvoro A, Gupte A A, Xia X, Baxter J D, Webb P and Hsueh W A

  J Biol Chem; 2011 Sep 2. 286 (35):30723-30731.

• Platelet Mitochondrial Dysfunction is Evident in Type 2 Diabetes in Association with Modifications of Mitochondrial Anti-Oxidant Stress Proteins

  Avila C, Huang R J, Stevens M V, Aponte A M, Tripodi D, Kim K Y and Sack M N

  Exp Clin Endocrinol Diabetes. 2011 Sep 15; [Epub ahead of print].

• SIRT3 attenuates palmitate-induced ROS production and inflammation in proximal tubular cells

  Koyama T, Kume S, Koya D, Araki S I, Isshiki K, Chin-Kanasaki M, Sugimoto T, Haneda M, Sugaya T, Kashiwagi A, Maegawa H and Uzu T

  Free Radic Biol Med; 2011 Sep 15. 51 (6):1258-1267.

• The PGC-1{alpha}-related coactivator promotes mitochondrial and myogenic adaptations in C2C12 myotubes

  Philp A, Belew M Y, Evans A, Pham D, Sivia I, Chen A, Schenk S and Baar K

  Am J Physiol Regul Integr Comp Physiol; 2011 Oct 1. 301 (4):R864-872.

• The differential role of hif1beta/arnt and the hypoxic response in adipose function, fibrosis, and inflammation

  Lee K Y, Gesta S, Boucher J, Wang X L and Kahn C R

  Cell Metab; 2011 Oct 5. 14 (4):491-503.

• Orexin is required for brown adipose tissue development, differentiation, and function

  Sellayah D, Bharaj P and Sikder D

  Cell Metab; 2011 Oct 5. 14 (4):478-490.

• Mitochondrial Complex III ROS Regulate Adipocyte Differentiation

  Tormos K V, Anso E, Hamanaka R B, Eisenbart J, Joseph J, Kalyanaraman B and Chandel N S

  Cell Metab; 2011 Oct 5. 14 (4):537-544.

• The Transcriptional Coactivators, PGC-1? and ?, Cooperate to Maintain Cardiac Mitochondrial Function During the Early Stages of Insulin Resistance

  Mitra R, Nogee D P, Zechner J F, Yea K, Gierasch C M, Kovacs A, Medeiros D M, Kelly D P and Duncan J G

  J Mol Cell Cardiol. 2011 Oct 20; [Epub ahead of print].

• Beta-Cell Uncoupling Protein 2 Regulates Reactive Oxygen Species Production, Which Influences Both Insulin and Glucagon Secretion

  Robson-Doucette C A, Sultan S, Allister E M, Wikstrom J D, Koshkin V, Bhatacharjee A, Prentice K J, Sereda S B, Shirihai O S and Wheeler M B

  Diabetes; 2011 Nov 1. 60 (11):2710-2719.

• High Glucose Induces Mitochondrial Morphology and Metabolic Changes in Retinal Pericytes

  Trudeau K, Molina A J and Roy S

  Invest Ophthalmol Vis Sci; 2011 Nov 7. 52 (12):8657-64.

• NCoR1 Is a Conserved Physiological Modulator of Muscle Mass and Oxidative Function

  Yamamoto H, Williams E G, Mouchiroud L, Canto C, Fan W, Downes M, Heligon C, Barish G D, Desvergne B, Evans R M, Schoonjans K and Auwerx J

  Cell; 2011 Nov 11. 147 (4):827-839.

• Fatty Acids Suppress Autophagic Turnover in beta-Cells

  Las G, Serada S B, Wikstrom J D, Twig G and Shirihai O S

  J Biol Chem; 2011 Dec 9. 286 (49):42534-42544.

• Galactose enhances oxidative metabolism and reveals mitochondrial dysfunction in human primary muscle cells

  Aguer C, Gambarotta D, Mailloux R J, Moffat C, Dent R, McPherson R and Harper M E

  PLoS One. 2011 Dec 15; [Epub ahead of print].

• The nuclear receptor PPARbeta/delta programs muscle glucose metabolism in cooperation with AMPK and MEF2

  Gan Z, Burkart-Hartman E M, Han D H, Finck B, Leone T C, Smith E Y, Ayala J E, Holloszy J and Kelly D P

  Genes Dev; 2011 Dec 15. 25 (24):2619-30.

• Human Pompe disease induced pluripotent stem cells for pathogenesis modeling, drug testing and disease marker identification

  Huang H P, Chen P H, Hwu W L, Chuang C Y, Chien Y H, Stone L, Chien C L, Li L T, Chiang S C, Chen H F, Ho H N, Chen C H and Kuo H C

  Hum Mol Genet; 2011 Dec 15. 20 (24):4851-64.

• Dietary supplementation with decaffeinated green coffee improves diet-induced insulin resistance and brain energy metabolism in mice

  Ho L, Varghese M, Wang J, Zhao W, Chen F, Knable L A, Ferruzzi M and Pasinetti G M

  Nutr Neurosci; 2012 Jan 1. 15 (1):37-45.

• Triiodothyronine induces UCP-1 expression and mitochondrial biogenesis in human adipocytes

  Lee J Y, Takahashi N, Yasubuchi M, Kim Y I, Hashizaki H, Kim M J, Sakamoto T, Goto T and Kawada T

  Am J Physiol Cell Physiol; 2012 Jan 1. 302 (2):C463-472.

• Crucial yet divergent roles of mitochondrial redox state in skeletal muscle vs. brown adipose tissue energetics

  Mailloux R J, Adjeitey C N, Xuan J Y and Harper M E

  FASEB J; 2012 Jan 1. 26 (1):363-75.

• Programming human pluripotent stem cells into white and brown adipocytes

  Ahfeldt T, Schinzel R T, Lee Y K, Hendrickson D, Kaplan A, Lum D H, Camahort R, Xia F, Shay J, Rhee E P, Clish C B, Deo R C, Shen T, Lau F H, Cowley A, Mowrer G, Al-Siddiqi H, Nahrendorf M, Musunuru K, Gerszten R E, Rinn J L and Cowan C A

  Nat Cell Biol; 2012 Jan 15. 14 (2):209-19.

• Reactive oxygen species stimulate insulin secretion in rat pancreatic islets: studies using mono-oleoyl-glycerol

  Saadeh M, Ferrante T C, Kane A, Shirihai O, Corkey B E and Deeney J T

  PLoS One. 2012 Jan 17; [Epub ahead of print].

• Dietary obesity-associated Hif1alpha activation in adipocytes restricts fatty acid oxidation and energy expenditure via suppression of the Sirt2-NAD+ system

  Krishnan J, Danzer C, Simka T, Ukropec J, Walter K M, Kumpf S, Mirtschink P, Ukropcova B, Gasperikova D, Pedrazzini T and Krek W

  Genes Dev; 2012 Feb 1. 26 (3):259-270.

• Cardiac natriuretic peptides act via p38 MAPK to induce the brown fat thermogenic program in mouse and human adipocytes

  Bordicchia M, Liu D, Amri E Z, Ailhaud G, Dessi-Fulgheri P, Zhang C, Takahashi N, Sarzani R and Collins S

  J Clin Invest. 2012 Feb 6; [Epub ahead of print].

• Altered mitochondrial function and metabolic inflexibility associated with loss of caveolin-1

  Wernstedt Asterholm I, Mundy D I, Weng J, Anderson R G and Scherer P E

  Cell Metab; 2012 Feb 8. 15 (2):171-185.

Technical Posters

Sort by: Title | Author | Date

• Mitochondrial dynamics play a key role in brown adipocyte activation

  Jakob D Wikstrom, Samuel B Sereda, Yaguang Si, Marc Liesa, Guy Las, Gilad Twig, Kiana Mahdaviani, Barbara Corkey, Barbara Cannon, Jan Neder

  Mitochondrial Physiology and Medicine
  September 7-11, 2011
  Woods Hole, MA

• Selective over expression of toll-like receptor 4 in skeletal muscle causes impaired adaptation to high-fat feeding

  Ryan McMillan, Yaru Wu, Kevin Voelker, John Kavanaugh, Kristin Wahlberg, Angela Anderson, Kim Haynie, Madlyn Frisard, and Matthew Hulver

  American Diabetes Association 71st Scientific Sessions
  June 24-28, 2011
  San Diego, CA

• Selective over expression of toll-like receptor 4 in skeletal muscle causes impaired adaptation to high-fat feeding

  Ryan McMillan, Yaru Wu, Kevin Voelker, John Kavanaugh, Kristin Wahlberg, Angela Anderson, Kim Haynie, Madlyn Frisard, and Matthew Hulver

  American Diabetes Association 71st Scientific Sessions
  June 24-28, 2011
  San Diego, CA

• Deletion of the Mitochondrial Transcription Factor A (TFAM) in Adipose Tissue Causes Mitochondrial Dysfunction and Protects Mice from Obesity and Insulin Resistance

  Vernochet, PhD, Cecile

  American Diabetes Association 71st Scientific Sessions
  June 24-28, 2011
  San Diego, CA

• The role of toll-like receptor 4 in mitochondrial function and reactive oxygen species (ROS) production in skeletal muscle

  Yaru Wu, Madlyn Frisard, Elike Shabrokh, Kevin Voelker, Ryan P McMilian, Matthew W Hulver

  Experimental Biology 2011
  April 9-13, 2011
  Washington, DC

• Effect of Diabetes Mellitus on Mitochondrial Oxygen Consumption in Peripheral Blood Mononuclear Cells

  Hartman M, Levy V, Xu G, Frame A, Caiano T, Kluge M, Tabit C, Dohadwala M, Shah A, Kocherla M, Levitt A, Kim B, Duess M, Shenouda S, Holbrook M, Hambu

  D-Cure's 5th Annual Symposium
  March 29-30, 2011
  Tel Aviv, Israel

• The CREB Coactivators, Crtc2 and Crtc3 Function as Exercise Mimetics in Skeletal Muscle

  Nelson E. Bruno, Antonio L. Ameilo, Mariam B. Lawani and Michael D. Conkright.

  Keystone Symposia on Type 2 Diabetes, Insulin Resistance and Metabolic Dysfunction
  January 13-15, 2011
  Keystone, CO

• HSP72 Preserves Insulin Sensitivity and Prevents Peripheral Tissue Lipotoxicity By Increasing Oxidative Metabolism in Skeletal Muscle

  DC Henstridge, CR Bruce, BG Drew, N Watson, J Chung, V Ribas, SW Beaven, MJ Watt, M Hargreaves, SL McGee, MA Febbraio, AL Hevener

  Keystone Symposia on Type 2 Diabetes, Insulin Resistance and Metabolic Dysfunction
  January 13-15, 2011
  Keystone, CO

• Class IIa Histone Deacetylases Contribute to Skeletal Muscle Transcriptional and Metabolic Remodeling and Oxidative Stress in Insulin Resistance

  Courtney Swinton, Shona Morrison, Tim Connor, Ken Walder, Greg R. Steinberg, Mark Hargreaves and Sean L. McGee

  Keystone Symposia on Type 2 Diabetes, Insulin Resistance and Metabolic Dysfunction
  January 13-15, 2011
  Keystone, CO

• Chronic Hyperglycemia-Induced Attenuation of Mitochondrial Reserve Capacity Mediates Mesangial Cell Dysfunction in Diabetes

  Balu K Chacko, Colin Reily, Gloria A Benavides, Michelle S. Johnson, and Victor M. Darley-Usmar

  Society for Free Radical Biology's 17th Annual Meeting
  November 17-20, 2010
  Orlando, FL

• Differential Regulation of Metabolic Pathways By Snitrosation in Endothelial Cells

  Anne R. Diers, Katarzyna A. Broniowska and Neil Hogg

  Society for Free Radical Biology's 17th Annual Meeting
  November 17-20, 2010
  Orlando, FL

• The Mesodermal Developmental Gene Tbx15 Regulates Triglyceride Accumulation and Mitochondria Respiration in Adipocytes

  Gesta, Stephane

  Obesity 2010
  October 11, 2010
  San Diego, CA

• Islet Respiratory Leak is Acutely Regulated by Nutrients and ROS and is Dysregulated in the Obese State

  Jakob D Wikstrom, Alvaro Elorza, Emma M Allister, Samuel B Sereda, Linsey Stiles, Andy Neilson, David A Ferrick, Michael B Wheeler, Shaoping Deng, Orian S Shirihai

  European Association for the Study of Diabetes Annual Meeting
  September 20-24, 2010
  Stockholm, Sweden

• Brown Adipocyte Activation is Characterized By a Wave of Mitochondrial Fission and Depolarization That is Dependent On β3 Receptor Stimulation and Drp1, and is Characterized By Complete, But Reversible, Arrest of Fusion

  Samuel B Sereda, Jakob D. Wikstrom, Yaguang Si, Gilad Twig, Marc Liesa, Guy Las, Cannon B, Nedergaard J, and Orian S. Shirihai

  Brown Adipose Tissue and Human Obesity Conference
  July 10, 2010
  Stockholm, Sweden

• Sirt3, a Novel Regulator of Mitochondrial Function and Insulin Signaling in Diabetes and Insulin Resistant States

  Jing, Enxuan

  ADA 70th Scientific Sessions
  April 26-28, 2010
  Orlando, FL

• The Homeobox Protein Prox1 is a Negative Modulator of ERRα/PGC-1α Bioenergetic Functions

  Alexis Charest-Marcotte, Catherine R. Dufour, Brian J. Wilson, Annie M. Tremblay, Lillian J. Eichner, Daniel H. Arlow, Vamsi K. Mootha and Vincent Gigu

  Nuclear Receptors: Development, Physiology and Disease
  March 21-26, 2010
  Keystone, CO

• Glucose Availability Modulates Mitochondrial Sources of NADPH in Skeletal Muscle Cells: Implications for Oxidative Stress

  Ryan J. Mailloux and Mary-Ellen Harper

  Charleston Conference on Mitochondria Physiology and Pathobiology
  November 16, 2009
  Isle of Palms, NC

• Novel Islet Respirometry Assay Reveals High Levels of Uncoupled Respiration in Islets

  Jakob Wikstrom, Alvaro Elorza, Emma M Allister, Samuel B Sereda, Linsey Stiles, Andy Neilson, David A Ferrick, Michael B Wheeler, Shaoping Deng, Orian S Shirihai

  Charleston Conference on Mitochondria Physiology and Pathobiology
  November 16, 2009
  Isle of Palms, NC

• Loss of mitochondrial fusion protein in the beta-cell leads to diabetes, obesity, and islet hyperplasias

  Molina, Anthony

  69th Scientific Sessions of the American Diabetes Association
  June 5-9, 2009
  New Orleans, LA

• [Beta]-Adrenergic and cAMP-Stimulated Mitochondrial Uncoupling in Human Adipocytes

  Einav Yehuda-Shnaidman, Naresh Kumar, Ben Buehrer, Sheila Collins

  69th Scientific Sessions of the American Diabetes Association
  June 5-9, 2009
  New Orleans, LA

• Metformin-Induced Inhibition of Mitochondrial Complex I Activity Stimulates Fatty Acid Oxidation and Glycolysis While Blunting Glucose Oxidation in C2C12 Myocytes

  Min Wu, Amy L. Swift, Rebecca Moran and David A. Ferrick

  Gordon Research Conference: Molecular & Cellular Bioenergetics
  June 17, 2007
  Andover, NH

• A Novel, Non-Radioactive Method for Measuring Fatty Acid Oxidation and Cellular Metabolism in C2C12 Myocytes

  Mark E. Rothenberg, Tina Patel, Chris Braun, Suzanne Armitstead, Kristie Lemire, Rebecca Moran, Craig Beeson, David A. Ferrick

  Endocrine Society 2006 Annual Meeting
  June 24, 2006
  Boston, MA

• Extracellular Flux as an Indicator of Cellular Metabolism

  Chris Braun, Mark Rothenberg, Suzanne Armitstead, Kristie Lemire, Diane Parslow, Tina Patel, and David Ferrick

  NAASO Annual Meeting
  October 15, 2005
  Vancouver, BC, CA

Webinars

Sort by: Title | Speaker | Date
• 

  Mitochondrial Bioenergetics in Diabetic Cardiomyopathy

  E. Dale Abel MD PhD, University of Utah School of Medicine
  December 14, 2011

• 

  Biochemical analysis in C. elegans as a model for metabolic disease and aging

  Laurent Mouchiroud, PhD , École Polytechnique Fédérale de Lausanne
  Riekelt H. Houtkooper, PhD, École Polytechnique Fédérale de Lausanne
  November 30, 2011

• 

  Using Reserve Capacity to Screen for Mitochondrial Toxicities and Biogenesis

  Craig C. Beeson, PhD, Medical University of South Carolina
  March 23, 2011

• 

  New dimensions in adipocyte metabolism: Using the XF to probe human and rodent adipocytes and adipose tissue

  Sheila Collins, PhD, Diabetes and Obesity Research Center, Sanford-Burnham Medical Research Institute
  Einav Yehuda-Shnaidman PhD, RD, The Hebrew University of Jerusalem
  February 9, 2011

• 

  Molecular Scaffolds KSR1/2: Implications for Obesity and Cancer

  Robert Lewis, PhD, University of Nebraska Cancer Center
  March 24, 2010

• 

  How to Measure Mitochondrial Function in Whole Islets. Sensing the nutrient sensor: measuring bioenergetic parameters from intact mouse and human islets of langerhans

  Orian Shirihai, MD, PhD, Boston University Medical Center
  July 16, 2009

• 

  The Bioenergetics of Obesity: New Approaches to Characterize Genes Affecting Energy Balance in Mouse Models

  Karen Reue, PhD, David Geffen School of Medicine at UCLA
  April 30, 2009

• 

  Tutorial: Measuring Bioenergetics in a Variety of Cell Types

  David Ferrick, PhD, CSO, Seahorse Bioscience
  July 17, 2008

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