Applications in Immunology

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App Notes & Tech Briefs

Bioenergetic analysis of suspension cells: hematopoietic stem cells and lymphocytes

A real time assay that quantifies the ATP and biosynthetic demands of immune cell proliferation, differentiation and effector function.

Normal suspension cells, such as hematopoietic cells and lymphocytes are responsible for supplying oxygen to the body and protecting the host. Cellular metabolism is markedly dynamic in immune responses during proliferation and immune effector functions...
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Publications

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HVCN1 modulates BCR signal strength via regulation of BCR-dependent generation of reactive oxygen species
Capasso M, Bhamrah M K, Henley T, Boyd R S, Langlais C, Cain K, Dinsdale D, Pulford K, Khan M, Musset B, Cherny V V, Morgan D, Gascoyne R D, Vigorito E, DeCoursey T E, MacLennan I C and Dyer M J
Nat Immunol; 2010 Mar 1. 11 (3):265-272.
Fast kinase domain-containing protein 3 is a mitochondrial protein essential for cellular respiration
Simarro M, Gimenez-Cassina A, Kedersha N, Lazaro J B, Adelmant G O, Marto J A, Rhee K, Tisdale S, Danial N, Benarafa C, Orduna A and Anderson P
Biochem Biophys Res Commun; 2010 Oct 22. 401 (3):440-446.
The Lkb1 metabolic sensor maintains haematopoietic stem cell survival
Gurumurthy S, Xie S Z, Alagesan B, Kim J, Yusuf R Z, Saez B, Tzatsos A, Ozsolak F, Milos P, Ferrari F, Park P J, Shirihai O S, Scadden D T and Bardeesy N
Nature; 2010 Dec 2. 468 (7324):659-663.
Expression, signaling proficiency, and stimulatory function of the NKG2D lymphocyte receptor in human cancer cells
Benitez A C, Dai Z, Mann H H, Reeves R S, Margineantu D H, Gooley T A, Groh V and Spies T
Proc Natl Acad Sci U S A; 2011 Mar 8. 108 (10):4081-4086.
Mitochondrial reactive oxygen species promote production of proinflammatory cytokines and are elevated in TNFR1-associated periodic syndrome (TRAPS)
Bulua A C, Simon A, Maddipati R, Pelletier M, Park H, Kim K Y, Sack M N, Kastner D L and Siegel R M
J Exp Med; 2011 Mar 14. 208 (3):519-533.
Glutathionylation acts as a control switch for uncoupling proteins UCP2 and UCP3
Mailloux R J, Seifert E L, Bouillaud F, Aguer C, Collins S and Harper M E
J Biol Chem; 2011 Jun 17. 286 (24):21865-21875.
The Transcription Factor Myc Controls Metabolic Reprogramming upon T Lymphocyte Activation
Wang R, Dillon C P, Shi L Z, Milasta S, Carter R, Finkelstein D, McCormick L L, Fitzgerald P, Chi H, Munger J and Green D R
Immunity; 2011 Dec 23. 35 (6):871-882.
Mitochondrial Respiratory Capacity Is a Critical Regulator of CD8(+) T Cell Memory Development
van der Windt G J, Everts B, Chang C H, Curtis J D, Freitas T C, Amiel E, Pearce E J and Pearce E L
Immunity; 2012 Jan 27. 36 (1):38-78.

Technical Posters

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Mitochondrial dysfunction and oxidative damage in Osteoarthritis
Christos Gavriilidis, Robert W Taylor, David A Young
British Society for Matrix Biology Autumn Meeting, Matrix signals! Cell-matrix interactions in health and disease
September 8-9, 2011
Newcastle, UK
Control of B Cell Activation and Metabolism by the voltage-Gated Proton Channel HVCN1
Capasso, Melania
Keystone Symposium Lymphocyte Activation and Gene Expression
January 3, 2010
Breckenridge, CO

Webinars

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Mitochondrial Respiratory Capacity Is a Critical Regulator of CD8+ T Cell Memory Development
Erika Pearce, PhD, Washington University School of Medicine
St. Louis, MO
February 14, 2012
XF Glycolysis Stress Test Assesses Glycolysis and Glycolytic Capacity in Cells
Min Wu, PhD, Seahorse Bioscience
August 30, 2011

Immunology deals with the physiological functioning of the immune system in states of both health and disease, malfunctions of the immune system in immunological disorders such as autoimmune diseases, hypersensitivities, immune deficiency, transplant rejection, and the physical, chemical and physiological characteristics of the components of the immune system in vitro, in situ, and in vivo.

Metabolic reprogramming emerged to be critical in adaptive immune response. Understanding cellular metabolism and the role of mitochondrial dysfunction in relation to immune system and immune response can generate new insights into how to combat immunological disease.

The XF Analyzer generates data that can provide new understanding in relation to immunology and mitochondrial dysfunction, by allowing the measurement of cellular metabolism in real-time, in a microplate. By measuring the oxygen consumption rate (OCR) - a measure of mitochondrial respiration; as well as the extracellular acidification rate (ECAR)—a measure of glycolysis, you will be provided with the most physiologically relevant in vitro measurement for your bioenergetic studies.