The power of real-time kinetics unlocks essential cellular bioenergetic data

The Seahorse Extracellular Flux (XF) Analyzer, and XF Stress Test Kits, make cellular bioenergetic studies simple, efficient and user-friendly. The first new in vitro metabolic measurement in 50 years, XF Analyzers non-invasively profile the metabolic activity of cells in minutes, offering scientists a physiologic cell based assay for determination of basal oxygen consumption, glycolysis rates, ATP turnover and respiratory capacity in a single experiment to assess mitochondrial dysfunction.

Discover why over 1200 scientists are using the XF Analyzer for a faster, better and more accurate measurement of real-time cellular bioenergetics.

By measuring the two major energy producing pathways of the cell simultaneously, mitochondrial respiration and glycolysis, scientists get the most physiologically relevant bioenergetic assay available, resulting in a better overall view of metabolism. The XF Analyzer also measures fatty acid oxidation and metabolism of glucose and amino acids for kinetic metabolic information.

No labels, radioactivity, dyes, trypsin, or permeabilization!

Adherent cells, primary cells, suspension cells and isolated mitochondria can be analyzed easily. XF Analyzers allow you to measure mitochondrial function and dysfunction in living cells with less setup and clean up than Clark electrode methods.

Measure mitochondrial respiration and glycolytic function in cells with Seahorse Stress Test Kits.

XF Cell Mito Stress Test Profile

Mito Stress Test

The fundamental parameters of mitochondrial function: basal respiration, ATP turnover, proton leak, and maximal respiration, or spare respiratory capacity.

XF Glycolysis Stress Test Profile

Glyco Stress Test

The fundamental parameters of cellular glycolysis: glycolysis, glycolytic capacity, and glycolytic reserve capacity.

By combining quality controlled reagents with real-time, label-free measurements provided by the XF Analyzer, the XF Cell Mito Stress Test Kit provides a complete mitochondrial stress profile; and the XF Glycolysis Stress Test Kit reveals a complete glycolytic profile of the cell; while dedicated software algorithms automatically calculate the key metrics, simplifying data analysis and interpretation.

Everything you need to measure mitochondrial dysfunction in cells

XF Cellular Bioenergetics

Measure cell metabolism in minutes in
a microplate with the easy-to-use XF
Analyzers and XF Assay Kits. Analyzing
XF96 Analyzer mitochondrial function has never been easier!

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XF Cell Mito Stress Test Kit

Mito Stress Test

Everything you need to measure mitochondrial function in cells.

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XF Glycolysis Stress Test Kit

Glyco Stress Test

Now it's easy to measure glycolytic function in cells.

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Webinar On-Demand

Mitochondrial Respiratory Capacity Is a Critical Regulator of CD8+ T Cell Memory Development

Erika <span>Pearce</span>, PhD

Erika Pearce, PhD,
Washington University School of Medicine
St. Louis, MO
February 14, 2012

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Customer Testimonial

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    Customer Testimonial

    Customer Testimonial

    Sean McGee, PhD
    Deakin University School
    of Medicine, Australia

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